Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host

Most lactobacilli produce extracellular polysaccharides that are considered to contribute to the probiotic effect of many strains. Lacticaseibacillus rhamnosus CNCM I-3690 is an anti-inflammatory strain able to counterbalance gut barrier dysfunction. In this study ten spontaneous variants of CNCM I-...

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Autores principales: Martín, R., Benítez-Cabello, A., Kulakauskas, S., Viana, M. V. C., Chamignon, C., Courtin, P., Carbonne, C., Chain, F., Pham, H. P., Derrien, Muriel, Bermúdez-Humarán, L. G., Chapot-Chartier, M. P., Smokvina, T., Langella, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104810/
https://www.ncbi.nlm.nih.gov/pubmed/37059733
http://dx.doi.org/10.1038/s41598-023-32116-3
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author Martín, R.
Benítez-Cabello, A.
Kulakauskas, S.
Viana, M. V. C.
Chamignon, C.
Courtin, P.
Carbonne, C.
Chain, F.
Pham, H. P.
Derrien, Muriel
Bermúdez-Humarán, L. G.
Chapot-Chartier, M. P.
Smokvina, T.
Langella, P.
author_facet Martín, R.
Benítez-Cabello, A.
Kulakauskas, S.
Viana, M. V. C.
Chamignon, C.
Courtin, P.
Carbonne, C.
Chain, F.
Pham, H. P.
Derrien, Muriel
Bermúdez-Humarán, L. G.
Chapot-Chartier, M. P.
Smokvina, T.
Langella, P.
author_sort Martín, R.
collection PubMed
description Most lactobacilli produce extracellular polysaccharides that are considered to contribute to the probiotic effect of many strains. Lacticaseibacillus rhamnosus CNCM I-3690 is an anti-inflammatory strain able to counterbalance gut barrier dysfunction. In this study ten spontaneous variants of CNCM I-3690 with different EPS-production were generated and characterized by their ropy phenotype, the quantification of the secreted EPS and genetic analysis. Amongst them, two were further analysed in vitro and in vivo: an EPS over-producer (7292) and a low-producer derivative of 7292 (7358, with similar EPS levels than the wild type (WT) strain). Our results showed that 7292 does not have anti-inflammatory profile in vitro, and lost the capacity to adhere to the colonic epithelial cells as well as the protective effect on the permeability. Finally, 7292 lost the protective effects of the WT strain in a murine model of gut dysfunction. Notably, strain 7292 was unable to stimulate goblet cell mucus production and colonic IL-10 production, all key features for the beneficial effect of the WT strain. Furthermore, transcriptome analysis of colonic samples from 7292-treated mice showed a down-regulation of anti-inflammatory genes. Altogether, our results point out that the increase of EPS production in CNCM I-3690 impairs its protective effects and highlight the importance of the correct EPS synthesis for the beneficial effects of this strain.
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spelling pubmed-101048102023-04-16 Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host Martín, R. Benítez-Cabello, A. Kulakauskas, S. Viana, M. V. C. Chamignon, C. Courtin, P. Carbonne, C. Chain, F. Pham, H. P. Derrien, Muriel Bermúdez-Humarán, L. G. Chapot-Chartier, M. P. Smokvina, T. Langella, P. Sci Rep Article Most lactobacilli produce extracellular polysaccharides that are considered to contribute to the probiotic effect of many strains. Lacticaseibacillus rhamnosus CNCM I-3690 is an anti-inflammatory strain able to counterbalance gut barrier dysfunction. In this study ten spontaneous variants of CNCM I-3690 with different EPS-production were generated and characterized by their ropy phenotype, the quantification of the secreted EPS and genetic analysis. Amongst them, two were further analysed in vitro and in vivo: an EPS over-producer (7292) and a low-producer derivative of 7292 (7358, with similar EPS levels than the wild type (WT) strain). Our results showed that 7292 does not have anti-inflammatory profile in vitro, and lost the capacity to adhere to the colonic epithelial cells as well as the protective effect on the permeability. Finally, 7292 lost the protective effects of the WT strain in a murine model of gut dysfunction. Notably, strain 7292 was unable to stimulate goblet cell mucus production and colonic IL-10 production, all key features for the beneficial effect of the WT strain. Furthermore, transcriptome analysis of colonic samples from 7292-treated mice showed a down-regulation of anti-inflammatory genes. Altogether, our results point out that the increase of EPS production in CNCM I-3690 impairs its protective effects and highlight the importance of the correct EPS synthesis for the beneficial effects of this strain. Nature Publishing Group UK 2023-04-14 /pmc/articles/PMC10104810/ /pubmed/37059733 http://dx.doi.org/10.1038/s41598-023-32116-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Martín, R.
Benítez-Cabello, A.
Kulakauskas, S.
Viana, M. V. C.
Chamignon, C.
Courtin, P.
Carbonne, C.
Chain, F.
Pham, H. P.
Derrien, Muriel
Bermúdez-Humarán, L. G.
Chapot-Chartier, M. P.
Smokvina, T.
Langella, P.
Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title_full Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title_fullStr Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title_full_unstemmed Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title_short Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host
title_sort over-production of exopolysaccharide by lacticaseibacillus rhamnosus cncm i-3690 strain cutbacks its beneficial effect on the host
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104810/
https://www.ncbi.nlm.nih.gov/pubmed/37059733
http://dx.doi.org/10.1038/s41598-023-32116-3
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