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Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene
AIM: Development of an amplification method for further investigation of HBV S gene variation patterns. BACKGROUND: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC). METHODS: This study was performed on ten p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105500/ https://www.ncbi.nlm.nih.gov/pubmed/37070108 |
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author | Mokhtari, Fedra Kaboosi, Hami Mohebbi, Seyed Reza Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza |
author_facet | Mokhtari, Fedra Kaboosi, Hami Mohebbi, Seyed Reza Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza |
author_sort | Mokhtari, Fedra |
collection | PubMed |
description | AIM: Development of an amplification method for further investigation of HBV S gene variation patterns. BACKGROUND: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC). METHODS: This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region. RESULTS: In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated. CONCLUSION: Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing. |
format | Online Article Text |
id | pubmed-10105500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-101055002023-04-16 Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene Mokhtari, Fedra Kaboosi, Hami Mohebbi, Seyed Reza Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza Gastroenterol Hepatol Bed Bench Brief Report AIM: Development of an amplification method for further investigation of HBV S gene variation patterns. BACKGROUND: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC). METHODS: This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region. RESULTS: In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated. CONCLUSION: Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing. Shaheed Beheshti University of Medical Sciences 2023 /pmc/articles/PMC10105500/ /pubmed/37070108 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Brief Report Mokhtari, Fedra Kaboosi, Hami Mohebbi, Seyed Reza Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title | Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title_full | Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title_fullStr | Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title_full_unstemmed | Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title_short | Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene |
title_sort | development and evaluation of a semi-nested pcr technique for amplification and determination of different surface gene variation patterns in patients with chronic hbv infection: development of an amplification method for hbv s gene |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105500/ https://www.ncbi.nlm.nih.gov/pubmed/37070108 |
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