Exploring the potential application of alternative nuclei in NMR based metabolomics

INTRODUCTION: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on (1)H over other NMR-active nuclei. OBJECTIVES: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. METHOD: Proton, carbon, phosphorus, and nitrogen-based NM...

Descripción completa

Detalles Bibliográficos
Autores principales: Sinclair, Georgia M., Oakes, Sophie M., Warden, Andrew C., Paten, Amy M., Jones, Oliver A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105680/
https://www.ncbi.nlm.nih.gov/pubmed/37060493
http://dx.doi.org/10.1007/s11306-023-02003-z
Descripción
Sumario:INTRODUCTION: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on (1)H over other NMR-active nuclei. OBJECTIVES: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. METHOD: Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue. RESULTS: Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments. (15)N NMR was found to not be sensitive enough for general metabolomic work. CONCLUSIONS: Alternative NMR active nuclei are useful for metabolomics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-023-02003-z.

Ejemplares similares