Exploring the potential application of alternative nuclei in NMR based metabolomics

INTRODUCTION: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on (1)H over other NMR-active nuclei. OBJECTIVES: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. METHOD: Proton, carbon, phosphorus, and nitrogen-based NM...

Descripción completa

Detalles Bibliográficos
Autores principales: Sinclair, Georgia M., Oakes, Sophie M., Warden, Andrew C., Paten, Amy M., Jones, Oliver A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105680/
https://www.ncbi.nlm.nih.gov/pubmed/37060493
http://dx.doi.org/10.1007/s11306-023-02003-z
_version_ 1785026262768549888
author Sinclair, Georgia M.
Oakes, Sophie M.
Warden, Andrew C.
Paten, Amy M.
Jones, Oliver A. H.
author_facet Sinclair, Georgia M.
Oakes, Sophie M.
Warden, Andrew C.
Paten, Amy M.
Jones, Oliver A. H.
author_sort Sinclair, Georgia M.
collection PubMed
description INTRODUCTION: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on (1)H over other NMR-active nuclei. OBJECTIVES: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. METHOD: Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue. RESULTS: Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments. (15)N NMR was found to not be sensitive enough for general metabolomic work. CONCLUSIONS: Alternative NMR active nuclei are useful for metabolomics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-023-02003-z.
format Online
Article
Text
id pubmed-10105680
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-101056802023-04-17 Exploring the potential application of alternative nuclei in NMR based metabolomics Sinclair, Georgia M. Oakes, Sophie M. Warden, Andrew C. Paten, Amy M. Jones, Oliver A. H. Metabolomics Original Article INTRODUCTION: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on (1)H over other NMR-active nuclei. OBJECTIVES: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. METHOD: Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue. RESULTS: Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments. (15)N NMR was found to not be sensitive enough for general metabolomic work. CONCLUSIONS: Alternative NMR active nuclei are useful for metabolomics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11306-023-02003-z. Springer US 2023-04-15 2023 /pmc/articles/PMC10105680/ /pubmed/37060493 http://dx.doi.org/10.1007/s11306-023-02003-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Sinclair, Georgia M.
Oakes, Sophie M.
Warden, Andrew C.
Paten, Amy M.
Jones, Oliver A. H.
Exploring the potential application of alternative nuclei in NMR based metabolomics
title Exploring the potential application of alternative nuclei in NMR based metabolomics
title_full Exploring the potential application of alternative nuclei in NMR based metabolomics
title_fullStr Exploring the potential application of alternative nuclei in NMR based metabolomics
title_full_unstemmed Exploring the potential application of alternative nuclei in NMR based metabolomics
title_short Exploring the potential application of alternative nuclei in NMR based metabolomics
title_sort exploring the potential application of alternative nuclei in nmr based metabolomics
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105680/
https://www.ncbi.nlm.nih.gov/pubmed/37060493
http://dx.doi.org/10.1007/s11306-023-02003-z
work_keys_str_mv AT sinclairgeorgiam exploringthepotentialapplicationofalternativenucleiinnmrbasedmetabolomics
AT oakessophiem exploringthepotentialapplicationofalternativenucleiinnmrbasedmetabolomics
AT wardenandrewc exploringthepotentialapplicationofalternativenucleiinnmrbasedmetabolomics
AT patenamym exploringthepotentialapplicationofalternativenucleiinnmrbasedmetabolomics
AT jonesoliverah exploringthepotentialapplicationofalternativenucleiinnmrbasedmetabolomics

Ejemplares similares