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Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana

BACKGROUND: The human host elicits specific immune responses after exposure to various life stages of the malaria parasite as well as components of mosquito saliva injected into the host during a mosquito bite. This study describes differences in IgG responses against antigens derived from the sporo...

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Autores principales: Kwapong, Sebastian Shine, Asare, Kwame Kumi, Kusi, Kwadwo Asamoah, Pappoe, Faustina, Ndam, Nicaise, Tahar, Rachida, Poinsignon, Anne, Amoah, Linda Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105943/
https://www.ncbi.nlm.nih.gov/pubmed/37061695
http://dx.doi.org/10.1186/s12936-023-04557-8
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author Kwapong, Sebastian Shine
Asare, Kwame Kumi
Kusi, Kwadwo Asamoah
Pappoe, Faustina
Ndam, Nicaise
Tahar, Rachida
Poinsignon, Anne
Amoah, Linda Eva
author_facet Kwapong, Sebastian Shine
Asare, Kwame Kumi
Kusi, Kwadwo Asamoah
Pappoe, Faustina
Ndam, Nicaise
Tahar, Rachida
Poinsignon, Anne
Amoah, Linda Eva
author_sort Kwapong, Sebastian Shine
collection PubMed
description BACKGROUND: The human host elicits specific immune responses after exposure to various life stages of the malaria parasite as well as components of mosquito saliva injected into the host during a mosquito bite. This study describes differences in IgG responses against antigens derived from the sporozoite (PfCSP), asexual stage parasite (PfEBA175) and the gametocyte (Pfs230), in addition to an Anopheles gambiae salivary gland antigen (gSG6-P1), in two communities in Ghana with similar blood stage malaria parasite prevalence. METHODS: This study used archived plasma samples collected from an earlier cross-sectional study that enrolled volunteers aged from 6 months to 70 years from Simiw, peri-urban community (N = 347) and Obom, rural community (N = 291). An archived thick and thin blood smear for microscopy was used for the estimation of Plasmodium parasite density and species and DNA extraction from blood spots and P. falciparum confirmation was performed using PCR. This study used the stored plasma samples to determine IgG antibody levels to P. falciparum and Anopheles salivary antigens using indirect ELISA. RESULTS: Individuals from Simiw had significantly higher levels of IgG against mosquito gSG6-P1 [median (95%CI)] [2.590 (2.452–2.783) ng/mL] compared to those from Obom [2.119 (1.957–2.345) ng/mL], p < 0.0001. Both IgG responses against Pfs230proC (p = 0.0006), and PfCSP (p = 0.002) were significantly lower in volunteers from Simiw compared to the participants from Obom. The seroprevalence of PfEBA-175.5R (p = 0.8613), gSG6-P1 (p = 0.0704), PfCSP (p = 0.7798) IgG were all similar in Obom and Simiw. However, Pfs230 seroprevalence was significantly higher at Obom compared to Simiw (p = 0.0006). Spearman correlation analysis showed no significant association between IgG responses against gSG6-P1, PfCSP, Pfs230proC and PfEBA-175.5R and parasite density at both Obom and Simiw (p > 0.05). CONCLUSION: In conclusion, the study showed that participants from Simiw had higher concentrations of circulating gSG6-P1 IgG antibodies but lower concentrations of P. falciparum antibodies, PfCSP IgG and Pfs230proC IgG compared to participants from Obom. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-023-04557-8.
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spelling pubmed-101059432023-04-17 Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana Kwapong, Sebastian Shine Asare, Kwame Kumi Kusi, Kwadwo Asamoah Pappoe, Faustina Ndam, Nicaise Tahar, Rachida Poinsignon, Anne Amoah, Linda Eva Malar J Research BACKGROUND: The human host elicits specific immune responses after exposure to various life stages of the malaria parasite as well as components of mosquito saliva injected into the host during a mosquito bite. This study describes differences in IgG responses against antigens derived from the sporozoite (PfCSP), asexual stage parasite (PfEBA175) and the gametocyte (Pfs230), in addition to an Anopheles gambiae salivary gland antigen (gSG6-P1), in two communities in Ghana with similar blood stage malaria parasite prevalence. METHODS: This study used archived plasma samples collected from an earlier cross-sectional study that enrolled volunteers aged from 6 months to 70 years from Simiw, peri-urban community (N = 347) and Obom, rural community (N = 291). An archived thick and thin blood smear for microscopy was used for the estimation of Plasmodium parasite density and species and DNA extraction from blood spots and P. falciparum confirmation was performed using PCR. This study used the stored plasma samples to determine IgG antibody levels to P. falciparum and Anopheles salivary antigens using indirect ELISA. RESULTS: Individuals from Simiw had significantly higher levels of IgG against mosquito gSG6-P1 [median (95%CI)] [2.590 (2.452–2.783) ng/mL] compared to those from Obom [2.119 (1.957–2.345) ng/mL], p < 0.0001. Both IgG responses against Pfs230proC (p = 0.0006), and PfCSP (p = 0.002) were significantly lower in volunteers from Simiw compared to the participants from Obom. The seroprevalence of PfEBA-175.5R (p = 0.8613), gSG6-P1 (p = 0.0704), PfCSP (p = 0.7798) IgG were all similar in Obom and Simiw. However, Pfs230 seroprevalence was significantly higher at Obom compared to Simiw (p = 0.0006). Spearman correlation analysis showed no significant association between IgG responses against gSG6-P1, PfCSP, Pfs230proC and PfEBA-175.5R and parasite density at both Obom and Simiw (p > 0.05). CONCLUSION: In conclusion, the study showed that participants from Simiw had higher concentrations of circulating gSG6-P1 IgG antibodies but lower concentrations of P. falciparum antibodies, PfCSP IgG and Pfs230proC IgG compared to participants from Obom. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-023-04557-8. BioMed Central 2023-04-15 /pmc/articles/PMC10105943/ /pubmed/37061695 http://dx.doi.org/10.1186/s12936-023-04557-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kwapong, Sebastian Shine
Asare, Kwame Kumi
Kusi, Kwadwo Asamoah
Pappoe, Faustina
Ndam, Nicaise
Tahar, Rachida
Poinsignon, Anne
Amoah, Linda Eva
Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title_full Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title_fullStr Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title_full_unstemmed Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title_short Mosquito bites and stage-specific antibody responses against Plasmodium falciparum in southern Ghana
title_sort mosquito bites and stage-specific antibody responses against plasmodium falciparum in southern ghana
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10105943/
https://www.ncbi.nlm.nih.gov/pubmed/37061695
http://dx.doi.org/10.1186/s12936-023-04557-8
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