Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment

Three-dimensional cell culture technology (3DCC) sits between two-dimensional cell culture (2DCC) and animal models and is widely used in oncology research. Compared to 2DCC, 3DCC allows cells to grow in a three-dimensional space, better simulating the in vivo growth environment of tumors, including...

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Autores principales: Zhang, Long, Liao, Weiqi, Chen, Shimin, Chen, Yukun, Cheng, Pengrui, Lu, Xinjun, Ma, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10106600/
https://www.ncbi.nlm.nih.gov/pubmed/37077835
http://dx.doi.org/10.3389/fonc.2023.1146477
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author Zhang, Long
Liao, Weiqi
Chen, Shimin
Chen, Yukun
Cheng, Pengrui
Lu, Xinjun
Ma, Yi
author_facet Zhang, Long
Liao, Weiqi
Chen, Shimin
Chen, Yukun
Cheng, Pengrui
Lu, Xinjun
Ma, Yi
author_sort Zhang, Long
collection PubMed
description Three-dimensional cell culture technology (3DCC) sits between two-dimensional cell culture (2DCC) and animal models and is widely used in oncology research. Compared to 2DCC, 3DCC allows cells to grow in a three-dimensional space, better simulating the in vivo growth environment of tumors, including hypoxia, nutrient concentration gradients, micro angiogenesis mimicism, and the interaction between tumor cells and the tumor microenvironment matrix. 3DCC has unparalleled advantages when compared to animal models, being more controllable, operable, and convenient. This review summarizes the comparison between 2DCC and 3DCC, as well as recent advances in different methods to obtain 3D models and their respective advantages and disadvantages.
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spelling pubmed-101066002023-04-18 Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment Zhang, Long Liao, Weiqi Chen, Shimin Chen, Yukun Cheng, Pengrui Lu, Xinjun Ma, Yi Front Oncol Oncology Three-dimensional cell culture technology (3DCC) sits between two-dimensional cell culture (2DCC) and animal models and is widely used in oncology research. Compared to 2DCC, 3DCC allows cells to grow in a three-dimensional space, better simulating the in vivo growth environment of tumors, including hypoxia, nutrient concentration gradients, micro angiogenesis mimicism, and the interaction between tumor cells and the tumor microenvironment matrix. 3DCC has unparalleled advantages when compared to animal models, being more controllable, operable, and convenient. This review summarizes the comparison between 2DCC and 3DCC, as well as recent advances in different methods to obtain 3D models and their respective advantages and disadvantages. Frontiers Media S.A. 2023-04-03 /pmc/articles/PMC10106600/ /pubmed/37077835 http://dx.doi.org/10.3389/fonc.2023.1146477 Text en Copyright © 2023 Zhang, Liao, Chen, Chen, Cheng, Lu and Ma https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zhang, Long
Liao, Weiqi
Chen, Shimin
Chen, Yukun
Cheng, Pengrui
Lu, Xinjun
Ma, Yi
Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title_full Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title_fullStr Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title_full_unstemmed Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title_short Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment
title_sort towards a new 3rs era in the construction of 3d cell culture models simulating tumor microenvironment
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10106600/
https://www.ncbi.nlm.nih.gov/pubmed/37077835
http://dx.doi.org/10.3389/fonc.2023.1146477
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