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The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor
The marine pathogen Vibrio vulnificus senses and responds to environmental stimuli via two chemosensory systems and 42–53 chemoreceptors. Here, we present an analysis of the V. vulnificus Aer2 chemoreceptor, VvAer2, which is the first V. vulnificus chemoreceptor to be characterized. VvAer2 is relate...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107281/ https://www.ncbi.nlm.nih.gov/pubmed/36420630 http://dx.doi.org/10.1111/mmi.15007 |
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author | Stuffle, Erwin C. Suzuki, Tise Orillard, Emilie Watts, Kylie J. |
author_facet | Stuffle, Erwin C. Suzuki, Tise Orillard, Emilie Watts, Kylie J. |
author_sort | Stuffle, Erwin C. |
collection | PubMed |
description | The marine pathogen Vibrio vulnificus senses and responds to environmental stimuli via two chemosensory systems and 42–53 chemoreceptors. Here, we present an analysis of the V. vulnificus Aer2 chemoreceptor, VvAer2, which is the first V. vulnificus chemoreceptor to be characterized. VvAer2 is related to the Aer2 receptors of other gammaproteobacteria, but uncharacteristically contains three PAS domains (PAS1‐3), rather than one or two. Using an E. coli chemotaxis hijack assay, we determined that VvAer2, like other Aer2 receptors, senses and responds to O(2). All three VvAer2 PAS domains bound pentacoordinate b‐type heme and exhibited similar O(2) affinities. PAS2 and PAS3 both stabilized O(2) via conserved Iβ‐Trp residues, but PAS1, which was easily oxidized in vitro, was unaffected by Iβ‐Trp replacement. Our results support a model in which PAS1 is largely dispensable for O(2)‐mediated signaling, whereas PAS2 modulates PAS3 signaling, and PAS3 signals to the downstream domains. Each PAS domain appeared to be positionally optimized, because PAS swapping caused altered signaling properties, and neither PAS1 nor PAS2 could replace PAS3. Our findings strengthen previous conclusions that Aer2 receptors are O(2) sensors, but with distinct N‐terminal domain arrangements that facilitate, modulate and tune responses based on environmental signals. |
format | Online Article Text |
id | pubmed-10107281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101072812023-04-18 The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor Stuffle, Erwin C. Suzuki, Tise Orillard, Emilie Watts, Kylie J. Mol Microbiol Research Articles The marine pathogen Vibrio vulnificus senses and responds to environmental stimuli via two chemosensory systems and 42–53 chemoreceptors. Here, we present an analysis of the V. vulnificus Aer2 chemoreceptor, VvAer2, which is the first V. vulnificus chemoreceptor to be characterized. VvAer2 is related to the Aer2 receptors of other gammaproteobacteria, but uncharacteristically contains three PAS domains (PAS1‐3), rather than one or two. Using an E. coli chemotaxis hijack assay, we determined that VvAer2, like other Aer2 receptors, senses and responds to O(2). All three VvAer2 PAS domains bound pentacoordinate b‐type heme and exhibited similar O(2) affinities. PAS2 and PAS3 both stabilized O(2) via conserved Iβ‐Trp residues, but PAS1, which was easily oxidized in vitro, was unaffected by Iβ‐Trp replacement. Our results support a model in which PAS1 is largely dispensable for O(2)‐mediated signaling, whereas PAS2 modulates PAS3 signaling, and PAS3 signals to the downstream domains. Each PAS domain appeared to be positionally optimized, because PAS swapping caused altered signaling properties, and neither PAS1 nor PAS2 could replace PAS3. Our findings strengthen previous conclusions that Aer2 receptors are O(2) sensors, but with distinct N‐terminal domain arrangements that facilitate, modulate and tune responses based on environmental signals. John Wiley and Sons Inc. 2022-12-04 2023-01 /pmc/articles/PMC10107281/ /pubmed/36420630 http://dx.doi.org/10.1111/mmi.15007 Text en © 2022 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Stuffle, Erwin C. Suzuki, Tise Orillard, Emilie Watts, Kylie J. The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title | The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title_full | The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title_fullStr | The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title_full_unstemmed | The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title_short | The Aer2 chemoreceptor from Vibrio vulnificus is a tri‐PAS‐heme oxygen sensor |
title_sort | aer2 chemoreceptor from vibrio vulnificus is a tri‐pas‐heme oxygen sensor |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107281/ https://www.ncbi.nlm.nih.gov/pubmed/36420630 http://dx.doi.org/10.1111/mmi.15007 |
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