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SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress

Cellular sugar starvation and/or energy deprivation serves as an important signaling cue for the live cells to trigger the necessary stress adaptation response. When exposed to cellular energy stress (ES) conditions, the plants reconfigure metabolic pathways and rebalance energy status while restric...

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Autores principales: Son, Seungmin, Im, Jong Hee, Ko, Jae‐Heung, Han, Kyung‐Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107498/
https://www.ncbi.nlm.nih.gov/pubmed/36478538
http://dx.doi.org/10.1111/nph.18597
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author Son, Seungmin
Im, Jong Hee
Ko, Jae‐Heung
Han, Kyung‐Hwan
author_facet Son, Seungmin
Im, Jong Hee
Ko, Jae‐Heung
Han, Kyung‐Hwan
author_sort Son, Seungmin
collection PubMed
description Cellular sugar starvation and/or energy deprivation serves as an important signaling cue for the live cells to trigger the necessary stress adaptation response. When exposed to cellular energy stress (ES) conditions, the plants reconfigure metabolic pathways and rebalance energy status while restricting vegetative organ growth. Despite the vital importance of this ES‐induced growth restriction, the regulatory mechanism underlying the response remains largely elusive in plants. Using plant cell‐ and whole plant‐based functional analyses coupled with extended genetic validation, we show that cellular ES‐activated SNF1‐related protein kinase 1 (SnRK1.1) directly interacts with and phosphorylates E2Fa transcription factor, a critical cell cycle regulator. Phosphorylation of E2Fa by SnRK1.1 leads to its proteasome‐mediated protein degradation, resulting in S‐phase repression and organ growth restriction. Our findings show that ES‐dependently activated SnRK1.1 adjusts cell proliferation and vegetative growth for plants to cope with constantly fluctuating environments.
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spelling pubmed-101074982023-04-18 SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress Son, Seungmin Im, Jong Hee Ko, Jae‐Heung Han, Kyung‐Hwan New Phytol Research Cellular sugar starvation and/or energy deprivation serves as an important signaling cue for the live cells to trigger the necessary stress adaptation response. When exposed to cellular energy stress (ES) conditions, the plants reconfigure metabolic pathways and rebalance energy status while restricting vegetative organ growth. Despite the vital importance of this ES‐induced growth restriction, the regulatory mechanism underlying the response remains largely elusive in plants. Using plant cell‐ and whole plant‐based functional analyses coupled with extended genetic validation, we show that cellular ES‐activated SNF1‐related protein kinase 1 (SnRK1.1) directly interacts with and phosphorylates E2Fa transcription factor, a critical cell cycle regulator. Phosphorylation of E2Fa by SnRK1.1 leads to its proteasome‐mediated protein degradation, resulting in S‐phase repression and organ growth restriction. Our findings show that ES‐dependently activated SnRK1.1 adjusts cell proliferation and vegetative growth for plants to cope with constantly fluctuating environments. John Wiley and Sons Inc. 2022-12-07 2023-02 /pmc/articles/PMC10107498/ /pubmed/36478538 http://dx.doi.org/10.1111/nph.18597 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Son, Seungmin
Im, Jong Hee
Ko, Jae‐Heung
Han, Kyung‐Hwan
SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title_full SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title_fullStr SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title_full_unstemmed SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title_short SNF1‐related protein kinase 1 represses Arabidopsis growth through post‐translational modification of E2Fa in response to energy stress
title_sort snf1‐related protein kinase 1 represses arabidopsis growth through post‐translational modification of e2fa in response to energy stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107498/
https://www.ncbi.nlm.nih.gov/pubmed/36478538
http://dx.doi.org/10.1111/nph.18597
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