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An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity

To cope with cold stress, plants have developed antioxidation strategies combined with osmoprotection by sugars. In potato (Solanum tuberosum) tubers, which are swollen stems, exposure to cold stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a signi...

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Autores principales: Teper‐Bamnolker, Paula, Roitman, Marina, Katar, Omri, Peleg, Noam, Aruchamy, Kalaivani, Suher, Shlomit, Doron‐Faigenboim, Adi, Leibman, Diana, Omid, Ayelet, Belausov, Eduard, Andersson, Mariette, Olsson, Niklas, Fält, Ann‐Sofie, Volpin, Hanne, Hofvander, Per, Gal‐On, Amit, Eshel, Dani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107833/
https://www.ncbi.nlm.nih.gov/pubmed/36448213
http://dx.doi.org/10.1111/tpj.16049
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author Teper‐Bamnolker, Paula
Roitman, Marina
Katar, Omri
Peleg, Noam
Aruchamy, Kalaivani
Suher, Shlomit
Doron‐Faigenboim, Adi
Leibman, Diana
Omid, Ayelet
Belausov, Eduard
Andersson, Mariette
Olsson, Niklas
Fält, Ann‐Sofie
Volpin, Hanne
Hofvander, Per
Gal‐On, Amit
Eshel, Dani
author_facet Teper‐Bamnolker, Paula
Roitman, Marina
Katar, Omri
Peleg, Noam
Aruchamy, Kalaivani
Suher, Shlomit
Doron‐Faigenboim, Adi
Leibman, Diana
Omid, Ayelet
Belausov, Eduard
Andersson, Mariette
Olsson, Niklas
Fält, Ann‐Sofie
Volpin, Hanne
Hofvander, Per
Gal‐On, Amit
Eshel, Dani
author_sort Teper‐Bamnolker, Paula
collection PubMed
description To cope with cold stress, plants have developed antioxidation strategies combined with osmoprotection by sugars. In potato (Solanum tuberosum) tubers, which are swollen stems, exposure to cold stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a significant part of the cold‐induced sweetening (CIS) response, by rapidly cleaving sucrose into hexoses and increasing osmoprotection. To discover alternative plant tissue pathways for coping with cold stress, we produced VInv‐knockout lines in two cultivars. Genome editing of VInv in ‘Désirée’ and ‘Brooke’ was done using stable and transient expression of CRISPR/Cas9 components, respectively. After storage at 4°C, sugar analysis indicated that the knockout lines showed low levels of CIS and maintained low acid invertase activity in storage. Surprisingly, the tuber parenchyma of vinv lines exhibited significantly reduced lipid peroxidation and reduced H(2)O(2) levels. Furthermore, whole plants of vinv lines exposed to cold stress without irrigation showed normal vigor, in contrast to WT plants, which wilted. Transcriptome analysis of vinv lines revealed upregulation of an osmoprotectant pathway and ethylene‐related genes during cold temperature exposure. Accordingly, higher expression of antioxidant‐related genes was detected after exposure to short and long cold storage. Sugar measurements showed an elevation of an alternative pathway in the absence of VInv activity, raising the raffinose pathway with increasing levels of myo‐inositol content as a cold tolerance response.
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spelling pubmed-101078332023-04-18 An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity Teper‐Bamnolker, Paula Roitman, Marina Katar, Omri Peleg, Noam Aruchamy, Kalaivani Suher, Shlomit Doron‐Faigenboim, Adi Leibman, Diana Omid, Ayelet Belausov, Eduard Andersson, Mariette Olsson, Niklas Fält, Ann‐Sofie Volpin, Hanne Hofvander, Per Gal‐On, Amit Eshel, Dani Plant J Original Articles To cope with cold stress, plants have developed antioxidation strategies combined with osmoprotection by sugars. In potato (Solanum tuberosum) tubers, which are swollen stems, exposure to cold stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a significant part of the cold‐induced sweetening (CIS) response, by rapidly cleaving sucrose into hexoses and increasing osmoprotection. To discover alternative plant tissue pathways for coping with cold stress, we produced VInv‐knockout lines in two cultivars. Genome editing of VInv in ‘Désirée’ and ‘Brooke’ was done using stable and transient expression of CRISPR/Cas9 components, respectively. After storage at 4°C, sugar analysis indicated that the knockout lines showed low levels of CIS and maintained low acid invertase activity in storage. Surprisingly, the tuber parenchyma of vinv lines exhibited significantly reduced lipid peroxidation and reduced H(2)O(2) levels. Furthermore, whole plants of vinv lines exposed to cold stress without irrigation showed normal vigor, in contrast to WT plants, which wilted. Transcriptome analysis of vinv lines revealed upregulation of an osmoprotectant pathway and ethylene‐related genes during cold temperature exposure. Accordingly, higher expression of antioxidant‐related genes was detected after exposure to short and long cold storage. Sugar measurements showed an elevation of an alternative pathway in the absence of VInv activity, raising the raffinose pathway with increasing levels of myo‐inositol content as a cold tolerance response. John Wiley and Sons Inc. 2022-12-22 2023-01 /pmc/articles/PMC10107833/ /pubmed/36448213 http://dx.doi.org/10.1111/tpj.16049 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Teper‐Bamnolker, Paula
Roitman, Marina
Katar, Omri
Peleg, Noam
Aruchamy, Kalaivani
Suher, Shlomit
Doron‐Faigenboim, Adi
Leibman, Diana
Omid, Ayelet
Belausov, Eduard
Andersson, Mariette
Olsson, Niklas
Fält, Ann‐Sofie
Volpin, Hanne
Hofvander, Per
Gal‐On, Amit
Eshel, Dani
An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title_full An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title_fullStr An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title_full_unstemmed An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title_short An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
title_sort alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107833/
https://www.ncbi.nlm.nih.gov/pubmed/36448213
http://dx.doi.org/10.1111/tpj.16049
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