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3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy

Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the opt...

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Detalles Bibliográficos
Autores principales: Verrier, Nicolas, Taddese, Asemare Mengistie, Abbessi, Riadh, Debailleul, Matthieu, Haeberlé, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107843/
https://www.ncbi.nlm.nih.gov/pubmed/36408663
http://dx.doi.org/10.1111/jmi.13160
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author Verrier, Nicolas
Taddese, Asemare Mengistie
Abbessi, Riadh
Debailleul, Matthieu
Haeberlé, Olivier
author_facet Verrier, Nicolas
Taddese, Asemare Mengistie
Abbessi, Riadh
Debailleul, Matthieu
Haeberlé, Olivier
author_sort Verrier, Nicolas
collection PubMed
description Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification.
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spelling pubmed-101078432023-04-18 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy Verrier, Nicolas Taddese, Asemare Mengistie Abbessi, Riadh Debailleul, Matthieu Haeberlé, Olivier J Microsc Technical Notes Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification. John Wiley and Sons Inc. 2022-11-30 2023-02 /pmc/articles/PMC10107843/ /pubmed/36408663 http://dx.doi.org/10.1111/jmi.13160 Text en © 2022 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Technical Notes
Verrier, Nicolas
Taddese, Asemare Mengistie
Abbessi, Riadh
Debailleul, Matthieu
Haeberlé, Olivier
3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title_full 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title_fullStr 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title_full_unstemmed 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title_short 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
title_sort 3d differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
topic Technical Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107843/
https://www.ncbi.nlm.nih.gov/pubmed/36408663
http://dx.doi.org/10.1111/jmi.13160
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