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3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the opt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107843/ https://www.ncbi.nlm.nih.gov/pubmed/36408663 http://dx.doi.org/10.1111/jmi.13160 |
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author | Verrier, Nicolas Taddese, Asemare Mengistie Abbessi, Riadh Debailleul, Matthieu Haeberlé, Olivier |
author_facet | Verrier, Nicolas Taddese, Asemare Mengistie Abbessi, Riadh Debailleul, Matthieu Haeberlé, Olivier |
author_sort | Verrier, Nicolas |
collection | PubMed |
description | Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification. |
format | Online Article Text |
id | pubmed-10107843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101078432023-04-18 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy Verrier, Nicolas Taddese, Asemare Mengistie Abbessi, Riadh Debailleul, Matthieu Haeberlé, Olivier J Microsc Technical Notes Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification. John Wiley and Sons Inc. 2022-11-30 2023-02 /pmc/articles/PMC10107843/ /pubmed/36408663 http://dx.doi.org/10.1111/jmi.13160 Text en © 2022 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Technical Notes Verrier, Nicolas Taddese, Asemare Mengistie Abbessi, Riadh Debailleul, Matthieu Haeberlé, Olivier 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title | 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title_full | 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title_fullStr | 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title_full_unstemmed | 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title_short | 3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
title_sort | 3d differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy |
topic | Technical Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107843/ https://www.ncbi.nlm.nih.gov/pubmed/36408663 http://dx.doi.org/10.1111/jmi.13160 |
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