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Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér

The economic and ecologically important genus Eucalyptus is rich in structurally diverse specialized metabolites. While some specialized metabolite classes are highly prevalent across the genus, the cyanogenic glucoside prunasin is only produced by c. 3% of species. To investigate the evolutionary m...

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Autores principales: Hansen, Cecilie Cetti, Sørensen, Mette, Bellucci, Matteo, Brandt, Wolfgang, Olsen, Carl Erik, Goodger, Jason Q. D., Woodrow, Ian E., Lindberg Møller, Birger, Neilson, Elizabeth H. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107851/
https://www.ncbi.nlm.nih.gov/pubmed/36305250
http://dx.doi.org/10.1111/nph.18581
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author Hansen, Cecilie Cetti
Sørensen, Mette
Bellucci, Matteo
Brandt, Wolfgang
Olsen, Carl Erik
Goodger, Jason Q. D.
Woodrow, Ian E.
Lindberg Møller, Birger
Neilson, Elizabeth H. J.
author_facet Hansen, Cecilie Cetti
Sørensen, Mette
Bellucci, Matteo
Brandt, Wolfgang
Olsen, Carl Erik
Goodger, Jason Q. D.
Woodrow, Ian E.
Lindberg Møller, Birger
Neilson, Elizabeth H. J.
author_sort Hansen, Cecilie Cetti
collection PubMed
description The economic and ecologically important genus Eucalyptus is rich in structurally diverse specialized metabolites. While some specialized metabolite classes are highly prevalent across the genus, the cyanogenic glucoside prunasin is only produced by c. 3% of species. To investigate the evolutionary mechanisms behind prunasin biosynthesis in Eucalyptus, we compared de novo assembled transcriptomes, together with online resources between cyanogenic and acyanogenic species. Identified genes were characterized in vivo and in vitro. Pathway characterization of cyanogenic Eucalyptus camphora and Eucalyptus yarraensis showed for the first time that the final glucosylation step from mandelonitrile to prunasin is catalyzed by a novel UDP‐glucosyltransferase UGT87. This step is typically catalyzed by a member of the UGT85 family, including in Eucalyptus cladocalyx. The upstream conversion of phenylalanine to mandelonitrile is catalyzed by three cytochrome P450 (CYP) enzymes from the CYP79, CYP706, and CYP71 families, as previously shown. Analysis of acyanogenic Eucalyptus species revealed the loss of different ortholog prunasin biosynthetic genes. The recruitment of UGTs from different families for prunasin biosynthesis in Eucalyptus demonstrates important pathway heterogeneities and unprecedented dynamic pathway evolution of chemical defense within a single genus. Overall, this study provides relevant insights into the tremendous adaptability of these long‐lived trees.
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spelling pubmed-101078512023-04-18 Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér Hansen, Cecilie Cetti Sørensen, Mette Bellucci, Matteo Brandt, Wolfgang Olsen, Carl Erik Goodger, Jason Q. D. Woodrow, Ian E. Lindberg Møller, Birger Neilson, Elizabeth H. J. New Phytol Research The economic and ecologically important genus Eucalyptus is rich in structurally diverse specialized metabolites. While some specialized metabolite classes are highly prevalent across the genus, the cyanogenic glucoside prunasin is only produced by c. 3% of species. To investigate the evolutionary mechanisms behind prunasin biosynthesis in Eucalyptus, we compared de novo assembled transcriptomes, together with online resources between cyanogenic and acyanogenic species. Identified genes were characterized in vivo and in vitro. Pathway characterization of cyanogenic Eucalyptus camphora and Eucalyptus yarraensis showed for the first time that the final glucosylation step from mandelonitrile to prunasin is catalyzed by a novel UDP‐glucosyltransferase UGT87. This step is typically catalyzed by a member of the UGT85 family, including in Eucalyptus cladocalyx. The upstream conversion of phenylalanine to mandelonitrile is catalyzed by three cytochrome P450 (CYP) enzymes from the CYP79, CYP706, and CYP71 families, as previously shown. Analysis of acyanogenic Eucalyptus species revealed the loss of different ortholog prunasin biosynthetic genes. The recruitment of UGTs from different families for prunasin biosynthesis in Eucalyptus demonstrates important pathway heterogeneities and unprecedented dynamic pathway evolution of chemical defense within a single genus. Overall, this study provides relevant insights into the tremendous adaptability of these long‐lived trees. John Wiley and Sons Inc. 2022-12-03 2023-02 /pmc/articles/PMC10107851/ /pubmed/36305250 http://dx.doi.org/10.1111/nph.18581 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research
Hansen, Cecilie Cetti
Sørensen, Mette
Bellucci, Matteo
Brandt, Wolfgang
Olsen, Carl Erik
Goodger, Jason Q. D.
Woodrow, Ian E.
Lindberg Møller, Birger
Neilson, Elizabeth H. J.
Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title_full Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title_fullStr Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title_full_unstemmed Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title_short Recruitment of distinct UDP‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér
title_sort recruitment of distinct udp‐glycosyltransferase families demonstrates dynamic evolution of chemical defense within eucalyptus l'hér
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107851/
https://www.ncbi.nlm.nih.gov/pubmed/36305250
http://dx.doi.org/10.1111/nph.18581
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