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Construction and evaluation of gRNA arrays for multiplex CRISPR‐Cas9

Endonuclease system CRISPR‐Cas9 represents a powerful toolbox for the budding yeast's Saccharomyces cerevisiae genome perturbation. The resulting double‐strand breaks are preferentially repaired via highly efficient homologous recombination, which subsequently leads to marker‐free genome editin...

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Detalles Bibliográficos
Autores principales:	Žun, Gašper, Doberšek, Katja, Petrovič, Uroš
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2023
Materias:	RESEARCH ARTICLE
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107897/ https://www.ncbi.nlm.nih.gov/pubmed/36536407 http://dx.doi.org/10.1002/yea.3833

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