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DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration

CRISPR/Cas9 genome editing technology can overcome many limitations of traditional breeding, offering enormous potential for crop improvement and food production. Although the direct delivery of Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) complexes to grapevine (Vitis vinifera) protoplasts...

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Detalles Bibliográficos
Autores principales: Najafi, Samaneh, Bertini, Edoardo, D’Incà, Erica, Fasoli, Marianna, Zenoni, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10108004/
https://www.ncbi.nlm.nih.gov/pubmed/37077374
http://dx.doi.org/10.1093/hr/uhac240