Cargando…

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

The multiple applications of super‐resolution microscopy have prompted the need for minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many fluorescent reporters display limitations (e.g., large and charged scaffolds, non‐specific binding) as building blocks for the cons...

Descripción completa

Detalles Bibliográficos
Autores principales: de Moliner, Fabio, Konieczna, Zuzanna, Mendive‐Tapia, Lorena, Saleeb, Rebecca S., Morris, Katie, Gonzalez‐Vera, Juan Antonio, Kaizuka, Takeshi, Grant, Seth G. N., Horrocks, Mathew H., Vendrell, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10108274/
https://www.ncbi.nlm.nih.gov/pubmed/36412996
http://dx.doi.org/10.1002/anie.202216231
Descripción
Sumario:The multiple applications of super‐resolution microscopy have prompted the need for minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many fluorescent reporters display limitations (e.g., large and charged scaffolds, non‐specific binding) as building blocks for the construction of fluorogenic peptides. Herein we have built a library of benzodiazole amino acids and systematically examined them as reporters for background‐free fluorescence microscopy. We have identified amine‐derivatized benzoselenadiazoles as scalable and photostable amino acids for the straightforward solid‐phase synthesis of fluorescent peptides. Benzodiazole amino acids retain the binding capabilities of bioactive peptides and display excellent signal‐to‐background ratios. Furthermore, we have demonstrated their application in peptide‐PAINT imaging of postsynaptic density protein‐95 nanoclusters in the synaptosomes from mouse brain tissues.