Cargando…
Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis
BACKGROUND: Serum exosome-based liquid biopsy has significant advantages for screening and diagnosing hepatocellular carcinoma (HCC). P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are novel small silencing RNAs that have been identified to function in cancer-related signalling path...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10108868/ https://www.ncbi.nlm.nih.gov/pubmed/37077942 http://dx.doi.org/10.2147/IJN.S398462 |
_version_ | 1785026931611140096 |
---|---|
author | Rui, Tao Wang, Kai Xiang, Aizhai Guo, Jufeng Tang, Ning Jin, Xin Lin, Yimou Liu, Jian Zhang, Xiaobing |
author_facet | Rui, Tao Wang, Kai Xiang, Aizhai Guo, Jufeng Tang, Ning Jin, Xin Lin, Yimou Liu, Jian Zhang, Xiaobing |
author_sort | Rui, Tao |
collection | PubMed |
description | BACKGROUND: Serum exosome-based liquid biopsy has significant advantages for screening and diagnosing hepatocellular carcinoma (HCC). P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are novel small silencing RNAs that have been identified to function in cancer-related signalling pathways. However, studies on the presence of piRNAs in serum exosomes from HCC patients and their diagnostic values in HCC are not well reported. Our aim is to validate serum exosome-derived piRNAs as the valuable component of liquid biopsy for diagnosing HCC. METHODS: We used small RNA (sRNA) sequencing to profile piRNAs from serum exosomes and describe the base distribution characteristics of serum exosome-derived piRNAs. Serum exosomes from 125 HCC patients and 44 nontumor donors were included in this study. RESULTS: We found that piRNAs were components of serum exosomes from HCC patients. A total of 253 differentially expressed serum exosome-derived piRNAs were screened from HCC compared with the piRNAs from nontumor donors. Serum exosome-derived piRNAs from HCC displayed a distinctive base distribution. To further confirm the potential diagnostic value of serum exosome-derived piRNAs in HCC, we detected the levels of the top 5 upregulated piRNAs in our Chinese cohort. The training set and validation set both showed that all 5 piRNAs were dramatically increased in the serum exosomes from HCC compared with the piRNAs from non-tumour donors. The piRNAs could strongly identify HCC patients from non-tumour donors according to the area under the receiver operating characteristic (AUROC) model. Additionally, the piRNAs could also present significant values for the diagnosis of HCC with low tumour burden. CONCLUSION: piRNAs enriched the components of serum exosomes from HCC and could serve as promising biomarkers for HCC diagnosis. |
format | Online Article Text |
id | pubmed-10108868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-101088682023-04-18 Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis Rui, Tao Wang, Kai Xiang, Aizhai Guo, Jufeng Tang, Ning Jin, Xin Lin, Yimou Liu, Jian Zhang, Xiaobing Int J Nanomedicine Original Research BACKGROUND: Serum exosome-based liquid biopsy has significant advantages for screening and diagnosing hepatocellular carcinoma (HCC). P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are novel small silencing RNAs that have been identified to function in cancer-related signalling pathways. However, studies on the presence of piRNAs in serum exosomes from HCC patients and their diagnostic values in HCC are not well reported. Our aim is to validate serum exosome-derived piRNAs as the valuable component of liquid biopsy for diagnosing HCC. METHODS: We used small RNA (sRNA) sequencing to profile piRNAs from serum exosomes and describe the base distribution characteristics of serum exosome-derived piRNAs. Serum exosomes from 125 HCC patients and 44 nontumor donors were included in this study. RESULTS: We found that piRNAs were components of serum exosomes from HCC patients. A total of 253 differentially expressed serum exosome-derived piRNAs were screened from HCC compared with the piRNAs from nontumor donors. Serum exosome-derived piRNAs from HCC displayed a distinctive base distribution. To further confirm the potential diagnostic value of serum exosome-derived piRNAs in HCC, we detected the levels of the top 5 upregulated piRNAs in our Chinese cohort. The training set and validation set both showed that all 5 piRNAs were dramatically increased in the serum exosomes from HCC compared with the piRNAs from non-tumour donors. The piRNAs could strongly identify HCC patients from non-tumour donors according to the area under the receiver operating characteristic (AUROC) model. Additionally, the piRNAs could also present significant values for the diagnosis of HCC with low tumour burden. CONCLUSION: piRNAs enriched the components of serum exosomes from HCC and could serve as promising biomarkers for HCC diagnosis. Dove 2023-04-13 /pmc/articles/PMC10108868/ /pubmed/37077942 http://dx.doi.org/10.2147/IJN.S398462 Text en © 2023 Rui et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Rui, Tao Wang, Kai Xiang, Aizhai Guo, Jufeng Tang, Ning Jin, Xin Lin, Yimou Liu, Jian Zhang, Xiaobing Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title | Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title_full | Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title_fullStr | Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title_full_unstemmed | Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title_short | Serum Exosome-Derived piRNAs Could Be Promising Biomarkers for HCC Diagnosis |
title_sort | serum exosome-derived pirnas could be promising biomarkers for hcc diagnosis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10108868/ https://www.ncbi.nlm.nih.gov/pubmed/37077942 http://dx.doi.org/10.2147/IJN.S398462 |
work_keys_str_mv | AT ruitao serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT wangkai serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT xiangaizhai serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT guojufeng serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT tangning serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT jinxin serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT linyimou serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT liujian serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis AT zhangxiaobing serumexosomederivedpirnascouldbepromisingbiomarkersforhccdiagnosis |