The State of the Hemostasis System under Conditions of Immobilization Stress against the Background of Experimental Metabolic Syndrome

Abstract—The state of the hemostasis system under conditions of immobilization stress against the background of metabolic syndrome (MS) in male Wistar rats was studied. Two series of experiments were conducted using single and multiple immobilization effects. It was shown that the development of MS with maintenance of rats on a high-calorie diet led to a decrease in the anticoagulant-fibrinolytic and antiplatelet potential of blood. In the plasma of intact rats subjected to both single and multiple immobilization, an increase in blood clotting and a decrease in fibrinolysis were found. At the same time, the use of multiple immobilization in comparison with single immobilization contributed to a more pronounced increase in platelet aggregation. The combination of both single and multiple immobilization and MS in rats caused a significant decrease in fibrinolysis, the anticoagulant activity of blood and increased platelet aggregation. However, with repeated immobilization exposure, an even more significant increase in ADP-dependent platelet aggregation was observed. A comparative analysis of the indicators of primary and plasma hemostasis under these experimental conditions allows us to conclude that immobilization, regardless of its duration, aggravates the state of hypercoagulation in rats with MS. Moreover, repeated immobilization stress on the background of metabolic disorders leads to more pronounced hypercoagulation shifts due to an 2-fold increase in platelet aggregation compared with intact animals.