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Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis
Somatosensory neurons extend enormous peripheral axons to the skin, where they detect diverse environmental stimuli. Somatosensory peripheral axons are easily damaged due to their small caliber and superficial location. Axonal damage results in Wallerian degeneration, creating vast quantities of cel...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110399/ https://www.ncbi.nlm.nih.gov/pubmed/36876992 http://dx.doi.org/10.1242/dmm.049911 |
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author | Peterman, Eric Quitevis, Elgene J. A. Black, Erik C. Horton, Emma C. Aelmore, Rune L. White, Ethan Sagasti, Alvaro Rasmussen, Jeffrey P. |
author_facet | Peterman, Eric Quitevis, Elgene J. A. Black, Erik C. Horton, Emma C. Aelmore, Rune L. White, Ethan Sagasti, Alvaro Rasmussen, Jeffrey P. |
author_sort | Peterman, Eric |
collection | PubMed |
description | Somatosensory neurons extend enormous peripheral axons to the skin, where they detect diverse environmental stimuli. Somatosensory peripheral axons are easily damaged due to their small caliber and superficial location. Axonal damage results in Wallerian degeneration, creating vast quantities of cellular debris that phagocytes must remove to maintain organ homeostasis. The cellular mechanisms that ensure efficient clearance of axon debris from stratified adult skin are unknown. Here, we established zebrafish scales as a tractable model to study axon degeneration in the adult epidermis. Using this system, we demonstrated that skin-resident immune cells known as Langerhans cells engulf the majority of axon debris. In contrast to immature skin, adult keratinocytes did not significantly contribute to debris removal, even in animals lacking Langerhans cells. Our study establishes a powerful new model for studying Wallerian degeneration and identifies a new function for Langerhans cells in maintenance of adult skin homeostasis following injury. These findings have important implications for pathologies that trigger somatosensory axon degeneration. |
format | Online Article Text |
id | pubmed-10110399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-101103992023-04-18 Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis Peterman, Eric Quitevis, Elgene J. A. Black, Erik C. Horton, Emma C. Aelmore, Rune L. White, Ethan Sagasti, Alvaro Rasmussen, Jeffrey P. Dis Model Mech Research Article Somatosensory neurons extend enormous peripheral axons to the skin, where they detect diverse environmental stimuli. Somatosensory peripheral axons are easily damaged due to their small caliber and superficial location. Axonal damage results in Wallerian degeneration, creating vast quantities of cellular debris that phagocytes must remove to maintain organ homeostasis. The cellular mechanisms that ensure efficient clearance of axon debris from stratified adult skin are unknown. Here, we established zebrafish scales as a tractable model to study axon degeneration in the adult epidermis. Using this system, we demonstrated that skin-resident immune cells known as Langerhans cells engulf the majority of axon debris. In contrast to immature skin, adult keratinocytes did not significantly contribute to debris removal, even in animals lacking Langerhans cells. Our study establishes a powerful new model for studying Wallerian degeneration and identifies a new function for Langerhans cells in maintenance of adult skin homeostasis following injury. These findings have important implications for pathologies that trigger somatosensory axon degeneration. The Company of Biologists Ltd 2023-04-03 /pmc/articles/PMC10110399/ /pubmed/36876992 http://dx.doi.org/10.1242/dmm.049911 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Peterman, Eric Quitevis, Elgene J. A. Black, Erik C. Horton, Emma C. Aelmore, Rune L. White, Ethan Sagasti, Alvaro Rasmussen, Jeffrey P. Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title | Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title_full | Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title_fullStr | Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title_full_unstemmed | Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title_short | Zebrafish cutaneous injury models reveal that Langerhans cells engulf axonal debris in adult epidermis |
title_sort | zebrafish cutaneous injury models reveal that langerhans cells engulf axonal debris in adult epidermis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110399/ https://www.ncbi.nlm.nih.gov/pubmed/36876992 http://dx.doi.org/10.1242/dmm.049911 |
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