Extending fluorescence anisotropy to large complexes using reversibly switchable proteins

The formation of macromolecular complexes can be measured by detection of changes in rotational mobility using time-resolved fluorescence anisotropy. However, this method is limited to relatively small molecules (~0.1–30 kDa), excluding the majority of the human proteome and its complexes. We descri...

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Detalles Bibliográficos
Autores principales: Volpato, Andrea, Ollech, Dirk, Alvelid, Jonatan, Damenti, Martina, Müller, Barbara, York, Andrew G, Ingaramo, Maria, Testa, Ilaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110461/
https://www.ncbi.nlm.nih.gov/pubmed/36217028
http://dx.doi.org/10.1038/s41587-022-01489-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110461/
https://www.ncbi.nlm.nih.gov/pubmed/36217028
http://dx.doi.org/10.1038/s41587-022-01489-7

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