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Spatial mapping of the total transcriptome by in situ polyadenylation

Spatial transcriptomics reveals the spatial context of gene expression, but current methods are limited to assaying polyadenylated (A-tailed) RNA transcripts. Here we demonstrate that enzymatic in situ polyadenylation of RNA enables detection of the full spectrum of RNAs, expanding the scope of sequ...

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Detalles Bibliográficos
Autores principales:	McKellar, David W., Mantri, Madhav, Hinchman, Meleana M., Parker, John S. L., Sethupathy, Praveen, Cosgrove, Benjamin D., De Vlaminck, Iwijn
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group US 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110464/ https://www.ncbi.nlm.nih.gov/pubmed/36329320 http://dx.doi.org/10.1038/s41587-022-01517-6

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