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Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding

Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics for food and forage. Our Africa-led plant genom...

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Autores principales: Njaci, Isaac, Waweru, Bernice, Kamal, Nadia, Muktar, Meki Shehabu, Fisher, David, Gundlach, Heidrun, Muli, Collins, Muthui, Lucy, Maranga, Mary, Kiambi, Davies, Maass, Brigitte L., Emmrich, Peter M. F., Domelevo Entfellner, Jean-Baka, Spannagl, Manuel, Chapman, Mark A., Shorinola, Oluwaseyi, Jones, Chris S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110558/
https://www.ncbi.nlm.nih.gov/pubmed/37069152
http://dx.doi.org/10.1038/s41467-023-37489-7
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author Njaci, Isaac
Waweru, Bernice
Kamal, Nadia
Muktar, Meki Shehabu
Fisher, David
Gundlach, Heidrun
Muli, Collins
Muthui, Lucy
Maranga, Mary
Kiambi, Davies
Maass, Brigitte L.
Emmrich, Peter M. F.
Domelevo Entfellner, Jean-Baka
Spannagl, Manuel
Chapman, Mark A.
Shorinola, Oluwaseyi
Jones, Chris S.
author_facet Njaci, Isaac
Waweru, Bernice
Kamal, Nadia
Muktar, Meki Shehabu
Fisher, David
Gundlach, Heidrun
Muli, Collins
Muthui, Lucy
Maranga, Mary
Kiambi, Davies
Maass, Brigitte L.
Emmrich, Peter M. F.
Domelevo Entfellner, Jean-Baka
Spannagl, Manuel
Chapman, Mark A.
Shorinola, Oluwaseyi
Jones, Chris S.
author_sort Njaci, Isaac
collection PubMed
description Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics for food and forage. Our Africa-led plant genome collaboration produces a high-quality chromosome-scale assembly of the lablab genome. Our assembly highlights the genome organisation of the trypsin inhibitor genes - an important anti-nutritional factor in lablab. We also re-sequence cultivated and wild lablab accessions from Africa confirming two domestication events. Finally, we examine the genetic and phenotypic diversity in a comprehensive lablab germplasm collection and identify genomic loci underlying variation of important agronomic traits in lablab. The genomic data generated here provide a valuable resource for lablab improvement. Our inclusive collaborative approach also presents an example that can be explored by other researchers sequencing indigenous crops, particularly from low and middle-income countries (LMIC).
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spelling pubmed-101105582023-04-19 Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding Njaci, Isaac Waweru, Bernice Kamal, Nadia Muktar, Meki Shehabu Fisher, David Gundlach, Heidrun Muli, Collins Muthui, Lucy Maranga, Mary Kiambi, Davies Maass, Brigitte L. Emmrich, Peter M. F. Domelevo Entfellner, Jean-Baka Spannagl, Manuel Chapman, Mark A. Shorinola, Oluwaseyi Jones, Chris S. Nat Commun Article Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics for food and forage. Our Africa-led plant genome collaboration produces a high-quality chromosome-scale assembly of the lablab genome. Our assembly highlights the genome organisation of the trypsin inhibitor genes - an important anti-nutritional factor in lablab. We also re-sequence cultivated and wild lablab accessions from Africa confirming two domestication events. Finally, we examine the genetic and phenotypic diversity in a comprehensive lablab germplasm collection and identify genomic loci underlying variation of important agronomic traits in lablab. The genomic data generated here provide a valuable resource for lablab improvement. Our inclusive collaborative approach also presents an example that can be explored by other researchers sequencing indigenous crops, particularly from low and middle-income countries (LMIC). Nature Publishing Group UK 2023-04-17 /pmc/articles/PMC10110558/ /pubmed/37069152 http://dx.doi.org/10.1038/s41467-023-37489-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Njaci, Isaac
Waweru, Bernice
Kamal, Nadia
Muktar, Meki Shehabu
Fisher, David
Gundlach, Heidrun
Muli, Collins
Muthui, Lucy
Maranga, Mary
Kiambi, Davies
Maass, Brigitte L.
Emmrich, Peter M. F.
Domelevo Entfellner, Jean-Baka
Spannagl, Manuel
Chapman, Mark A.
Shorinola, Oluwaseyi
Jones, Chris S.
Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title_full Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title_fullStr Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title_full_unstemmed Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title_short Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
title_sort chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110558/
https://www.ncbi.nlm.nih.gov/pubmed/37069152
http://dx.doi.org/10.1038/s41467-023-37489-7
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