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Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression
Prostate cancer (PCa) is the second most common malignancy in men, and the fifth leading cause of death worldwide. Mesenchymal stromal/stem cells (MSC) have been identified in PCa, although contradictory effects in malignant transformation and tumor progression have been described. Since umbilical c...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110723/ https://www.ncbi.nlm.nih.gov/pubmed/36806993 http://dx.doi.org/10.1007/s13577-023-00880-z |
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author | Sousa, André Coelho, Pedro Leite, Fernanda Teixeira, Catarina Rocha, Ana Catarina Santos, Inês Baylina, Pilar Fernandes, Ruben Soares, Raquel Costa, Raquel Gomes, Andreia |
author_facet | Sousa, André Coelho, Pedro Leite, Fernanda Teixeira, Catarina Rocha, Ana Catarina Santos, Inês Baylina, Pilar Fernandes, Ruben Soares, Raquel Costa, Raquel Gomes, Andreia |
author_sort | Sousa, André |
collection | PubMed |
description | Prostate cancer (PCa) is the second most common malignancy in men, and the fifth leading cause of death worldwide. Mesenchymal stromal/stem cells (MSC) have been identified in PCa, although contradictory effects in malignant transformation and tumor progression have been described. Since umbilical cord (UC) MSC and cord blood serum (CBS) are rich in numerous growth and anti-inflammatory factors, UC-MSC secretome and CBS are able to modulate tumor cell proliferation and survival as well as immunity and angiogenesis. In the present study, we address this relationship and investigate the influence of UC-MSC secretome and CBS on two human PCa cell lines (PC3 and LNCaP) and a normal epithelial prostate cell line (HPEpiC). Our results disclosed that upon exposure to UC-MSC-conditioned medium or CBS, both PC3 and LNCaP cells exhibited reduced viability, proliferation, and motility while non-malignant epithelial prostate cells were unaffected. These findings were corroborated by expression analysis of AKT/PI3K signaling pathway, p53 and interleukin genes. UC-MSC and CBS factors decreased the expression of growth-stimulating AKT and PI3K effectors and simultaneously up-regulated the expression of tumor-suppressor p53. Moreover, a more anti-inflammatory expression profile was found in both malignant PCa cell lines. Altogether, these results shed light into possible mechanisms by which UC-MSC and CBS reduce PCa progression, further reinforcing their potential use as novel therapeutic agents in PCa. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10110723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-101107232023-04-19 Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression Sousa, André Coelho, Pedro Leite, Fernanda Teixeira, Catarina Rocha, Ana Catarina Santos, Inês Baylina, Pilar Fernandes, Ruben Soares, Raquel Costa, Raquel Gomes, Andreia Hum Cell Research Article Prostate cancer (PCa) is the second most common malignancy in men, and the fifth leading cause of death worldwide. Mesenchymal stromal/stem cells (MSC) have been identified in PCa, although contradictory effects in malignant transformation and tumor progression have been described. Since umbilical cord (UC) MSC and cord blood serum (CBS) are rich in numerous growth and anti-inflammatory factors, UC-MSC secretome and CBS are able to modulate tumor cell proliferation and survival as well as immunity and angiogenesis. In the present study, we address this relationship and investigate the influence of UC-MSC secretome and CBS on two human PCa cell lines (PC3 and LNCaP) and a normal epithelial prostate cell line (HPEpiC). Our results disclosed that upon exposure to UC-MSC-conditioned medium or CBS, both PC3 and LNCaP cells exhibited reduced viability, proliferation, and motility while non-malignant epithelial prostate cells were unaffected. These findings were corroborated by expression analysis of AKT/PI3K signaling pathway, p53 and interleukin genes. UC-MSC and CBS factors decreased the expression of growth-stimulating AKT and PI3K effectors and simultaneously up-regulated the expression of tumor-suppressor p53. Moreover, a more anti-inflammatory expression profile was found in both malignant PCa cell lines. Altogether, these results shed light into possible mechanisms by which UC-MSC and CBS reduce PCa progression, further reinforcing their potential use as novel therapeutic agents in PCa. GRAPHICAL ABSTRACT: [Image: see text] Springer Nature Singapore 2023-02-19 2023 /pmc/articles/PMC10110723/ /pubmed/36806993 http://dx.doi.org/10.1007/s13577-023-00880-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Sousa, André Coelho, Pedro Leite, Fernanda Teixeira, Catarina Rocha, Ana Catarina Santos, Inês Baylina, Pilar Fernandes, Ruben Soares, Raquel Costa, Raquel Gomes, Andreia Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title | Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title_full | Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title_fullStr | Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title_full_unstemmed | Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title_short | Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
title_sort | impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110723/ https://www.ncbi.nlm.nih.gov/pubmed/36806993 http://dx.doi.org/10.1007/s13577-023-00880-z |
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