Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China

Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterized by cell...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Xiaojuan, Sun, Bo, Liu, Guifang, Wu, Yunjiao, Liu, Yao, Ji, Feng, Tao, Zexin, Xu, Aiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111730/
https://www.ncbi.nlm.nih.gov/pubmed/37072763
http://dx.doi.org/10.1186/s12985-023-02041-y
_version_ 1785027507296141312
author Lin, Xiaojuan
Sun, Bo
Liu, Guifang
Wu, Yunjiao
Liu, Yao
Ji, Feng
Tao, Zexin
Xu, Aiqiang
author_facet Lin, Xiaojuan
Sun, Bo
Liu, Guifang
Wu, Yunjiao
Liu, Yao
Ji, Feng
Tao, Zexin
Xu, Aiqiang
author_sort Lin, Xiaojuan
collection PubMed
description Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterized by cell fusion on C6/36 cell line. Its complete genome was 7611 nucleotides in length, with 71.4–90.4% similarities with other OMRV strains. Phylogenetic analysis based on complete genomes showed all OMRV-like strains can be divided into 3 groups with between-group distances ranging from 0.254 to 0.293. These results revealed that the OMRV isolate had high genetic diversity with those identified previously, and enriched the genetic information of family Totiviridae.
format Online
Article
Text
id pubmed-10111730
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-101117302023-04-19 Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China Lin, Xiaojuan Sun, Bo Liu, Guifang Wu, Yunjiao Liu, Yao Ji, Feng Tao, Zexin Xu, Aiqiang Virol J Brief Report Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterized by cell fusion on C6/36 cell line. Its complete genome was 7611 nucleotides in length, with 71.4–90.4% similarities with other OMRV strains. Phylogenetic analysis based on complete genomes showed all OMRV-like strains can be divided into 3 groups with between-group distances ranging from 0.254 to 0.293. These results revealed that the OMRV isolate had high genetic diversity with those identified previously, and enriched the genetic information of family Totiviridae. BioMed Central 2023-04-18 /pmc/articles/PMC10111730/ /pubmed/37072763 http://dx.doi.org/10.1186/s12985-023-02041-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Brief Report
Lin, Xiaojuan
Sun, Bo
Liu, Guifang
Wu, Yunjiao
Liu, Yao
Ji, Feng
Tao, Zexin
Xu, Aiqiang
Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title_full Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title_fullStr Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title_full_unstemmed Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title_short Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
title_sort genomic characterization of an omono river virus isolated from culex tritaeniorhynchus in eastern china
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111730/
https://www.ncbi.nlm.nih.gov/pubmed/37072763
http://dx.doi.org/10.1186/s12985-023-02041-y
work_keys_str_mv AT linxiaojuan genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT sunbo genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT liuguifang genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT wuyunjiao genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT liuyao genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT jifeng genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT taozexin genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina
AT xuaiqiang genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina

Ejemplares similares