T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells

BACKGROUND: RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging. RESULTS: We here describe a tagmentation-based, rRNA blocked, random hexamer prime...

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Detalles Bibliográficos
Autores principales: Gustafsson, Charlotte, Hauenstein, Julia, Frengen, Nicolai, Krstic, Aleksandra, Luc, Sidinh, Månsson, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111750/
https://www.ncbi.nlm.nih.gov/pubmed/37069502
http://dx.doi.org/10.1186/s12864-023-09279-4

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