Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus

The monoclonal antibody (mAb) IP5B11, which is used worldwide for the diagnosis of viral haemorrhagic septicaemia (VHS) in fish, reacts with all genotypes of VHS virus (VHSV). The mAb exceptionally also reacts with the carpione rhabdovirus (CarRV). Following next generation genome sequencing of CarR...

Descripción completa

Detalles Bibliográficos
Autores principales: Ito, Takafumi, Mekata, Tohru, Olesen, Niels Jørgen, Lorenzen, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111850/
https://www.ncbi.nlm.nih.gov/pubmed/37069579
http://dx.doi.org/10.1186/s13567-023-01166-w
_version_ 1785027531680776192
author Ito, Takafumi
Mekata, Tohru
Olesen, Niels Jørgen
Lorenzen, Niels
author_facet Ito, Takafumi
Mekata, Tohru
Olesen, Niels Jørgen
Lorenzen, Niels
author_sort Ito, Takafumi
collection PubMed
description The monoclonal antibody (mAb) IP5B11, which is used worldwide for the diagnosis of viral haemorrhagic septicaemia (VHS) in fish, reacts with all genotypes of VHS virus (VHSV). The mAb exceptionally also reacts with the carpione rhabdovirus (CarRV). Following next generation genome sequencing of CarRV and N protein sequence alignment including five kinds of fish novirhabdoviruses, the epitope recognized by mAb IP5B11 was identified. Dot blot analysis confirmed the epitope of mAb IP5B11 to be associated with the region N219 to N233 of the N protein of VHSV. Phylogenetic analysis identified CarRV as a new member of the fish novirhabdoviruses.
format Online
Article
Text
id pubmed-10111850
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-101118502023-04-19 Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus Ito, Takafumi Mekata, Tohru Olesen, Niels Jørgen Lorenzen, Niels Vet Res Short Report The monoclonal antibody (mAb) IP5B11, which is used worldwide for the diagnosis of viral haemorrhagic septicaemia (VHS) in fish, reacts with all genotypes of VHS virus (VHSV). The mAb exceptionally also reacts with the carpione rhabdovirus (CarRV). Following next generation genome sequencing of CarRV and N protein sequence alignment including five kinds of fish novirhabdoviruses, the epitope recognized by mAb IP5B11 was identified. Dot blot analysis confirmed the epitope of mAb IP5B11 to be associated with the region N219 to N233 of the N protein of VHSV. Phylogenetic analysis identified CarRV as a new member of the fish novirhabdoviruses. BioMed Central 2023-04-17 2023 /pmc/articles/PMC10111850/ /pubmed/37069579 http://dx.doi.org/10.1186/s13567-023-01166-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Ito, Takafumi
Mekata, Tohru
Olesen, Niels Jørgen
Lorenzen, Niels
Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title_full Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title_fullStr Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title_full_unstemmed Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title_short Epitope mapping of the monoclonal antibody IP5B11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
title_sort epitope mapping of the monoclonal antibody ip5b11 used for detection of viral haemorrhagic septicaemia virus facilitated by genome sequencing of carpione novirhabdovirus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111850/
https://www.ncbi.nlm.nih.gov/pubmed/37069579
http://dx.doi.org/10.1186/s13567-023-01166-w
work_keys_str_mv AT itotakafumi epitopemappingofthemonoclonalantibodyip5b11usedfordetectionofviralhaemorrhagicsepticaemiavirusfacilitatedbygenomesequencingofcarpionenovirhabdovirus
AT mekatatohru epitopemappingofthemonoclonalantibodyip5b11usedfordetectionofviralhaemorrhagicsepticaemiavirusfacilitatedbygenomesequencingofcarpionenovirhabdovirus
AT olesennielsjørgen epitopemappingofthemonoclonalantibodyip5b11usedfordetectionofviralhaemorrhagicsepticaemiavirusfacilitatedbygenomesequencingofcarpionenovirhabdovirus
AT lorenzenniels epitopemappingofthemonoclonalantibodyip5b11usedfordetectionofviralhaemorrhagicsepticaemiavirusfacilitatedbygenomesequencingofcarpionenovirhabdovirus

Ejemplares similares