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Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice

Clostridium butyricum (CB) is a spore-forming, gram-positive and obligate anaerobic rod bacterium. CB can modulate the composition of the gut microbiome and promote the growth of beneficial microbes in the intestine by generating short-chain fatty acids (SCFAs), which in turn protect against colitis...

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Autores principales: Pu, Wenfeng, Zhang, Hong, Zhang, Tao, Guo, Xiaoguang, Wang, Xiaoqing, Tang, Shaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111964/
https://www.ncbi.nlm.nih.gov/pubmed/37081884
http://dx.doi.org/10.3389/fimmu.2023.1004756
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author Pu, Wenfeng
Zhang, Hong
Zhang, Tao
Guo, Xiaoguang
Wang, Xiaoqing
Tang, Shaohui
author_facet Pu, Wenfeng
Zhang, Hong
Zhang, Tao
Guo, Xiaoguang
Wang, Xiaoqing
Tang, Shaohui
author_sort Pu, Wenfeng
collection PubMed
description Clostridium butyricum (CB) is a spore-forming, gram-positive and obligate anaerobic rod bacterium. CB can modulate the composition of the gut microbiome and promote the growth of beneficial microbes in the intestine by generating short-chain fatty acids (SCFAs), which in turn protect against colitis and prevents the formation of inflammatory-associated colorectal cancer (CRC) by ameliorating colon inflammatory processes. Yet, it remains unclear whether the culture and supernatant of CB could directly influence inflammatory CRC in mice. In this study, azoxymethane (AOM)+dextran sodium sulphate (DSS) was used to induce CRC model in C57BL/6 mice. Next, the serum levels of inflammatory cytokines, including interleukin-6 (IL-6), interleukin-10 (IL-10), and cytokines TNF-α, were measured and the pathohistological examination of the large intestine was performed. Both CB culture and supernatant were found to have anti-inflammatory properties. Subsequently, Western blot and Real-Time Quantitative PCR (RT-qPCR) revealed that CB and supernatant regulate the NF-κB/p65 pathway to inhibit the development and progression of inflammatory CRC in AOM+DSS-treated mice, which could be due to the high levels of butyric acid in the supernatant.
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spelling pubmed-101119642023-04-19 Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice Pu, Wenfeng Zhang, Hong Zhang, Tao Guo, Xiaoguang Wang, Xiaoqing Tang, Shaohui Front Immunol Immunology Clostridium butyricum (CB) is a spore-forming, gram-positive and obligate anaerobic rod bacterium. CB can modulate the composition of the gut microbiome and promote the growth of beneficial microbes in the intestine by generating short-chain fatty acids (SCFAs), which in turn protect against colitis and prevents the formation of inflammatory-associated colorectal cancer (CRC) by ameliorating colon inflammatory processes. Yet, it remains unclear whether the culture and supernatant of CB could directly influence inflammatory CRC in mice. In this study, azoxymethane (AOM)+dextran sodium sulphate (DSS) was used to induce CRC model in C57BL/6 mice. Next, the serum levels of inflammatory cytokines, including interleukin-6 (IL-6), interleukin-10 (IL-10), and cytokines TNF-α, were measured and the pathohistological examination of the large intestine was performed. Both CB culture and supernatant were found to have anti-inflammatory properties. Subsequently, Western blot and Real-Time Quantitative PCR (RT-qPCR) revealed that CB and supernatant regulate the NF-κB/p65 pathway to inhibit the development and progression of inflammatory CRC in AOM+DSS-treated mice, which could be due to the high levels of butyric acid in the supernatant. Frontiers Media S.A. 2023-04-04 /pmc/articles/PMC10111964/ /pubmed/37081884 http://dx.doi.org/10.3389/fimmu.2023.1004756 Text en Copyright © 2023 Pu, Zhang, Zhang, Guo, Wang and Tang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Pu, Wenfeng
Zhang, Hong
Zhang, Tao
Guo, Xiaoguang
Wang, Xiaoqing
Tang, Shaohui
Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title_full Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title_fullStr Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title_full_unstemmed Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title_short Inhibitory effects of Clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
title_sort inhibitory effects of clostridium butyricum culture and supernatant on inflammatory colorectal cancer in mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111964/
https://www.ncbi.nlm.nih.gov/pubmed/37081884
http://dx.doi.org/10.3389/fimmu.2023.1004756
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