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Longitudinal In Vivo Monitoring of Atheroprogression in Hypercholesterolemic Mice Using Photoacoustic Imaging

Background and Aim  The ability to recognize and monitor atherosclerotic lesion development using noninvasive imaging is crucial in preventive cardiology. The aim of the present study was to establish a protocol for longitudinal monitoring of plaque lipid, collagen, and macrophage burden as well as...

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Detalles Bibliográficos
Autores principales: Ferraro, Bartolo, Giustetto, Pierangela, Schengel, Olga, Weckbach, Ludwig T., Maegdefessel, Lars, Soehnlein, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113035/
https://www.ncbi.nlm.nih.gov/pubmed/36596447
http://dx.doi.org/10.1055/a-2005-8784
Descripción
Sumario:Background and Aim  The ability to recognize and monitor atherosclerotic lesion development using noninvasive imaging is crucial in preventive cardiology. The aim of the present study was to establish a protocol for longitudinal monitoring of plaque lipid, collagen, and macrophage burden as well as of endothelial permeability. Methods and Results  Photoacoustic signals derived from endogenous or exogenous dyes assessed in vivo, in plaques of albino Apoe (−/−) mice, correlated with lesion characteristics obtained after histomorphometric and immunofluorescence analyses, thus supporting the validity of our protocol. Using models of atheroprogression and regression, we could apply our imaging protocol to the longitudinal observation of atherosclerotic lesion characteristics in mice. Conclusions  The present study shows an innovative approach to assess arterial inflammation in a non-invasive fashion, applicable to longitudinal analyses of changes of atherosclerotic lesion composition. Such approach could prove important in the preclinical testing of therapeutic interventions in mice carrying pre-established lesions.