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Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits

Nonalcoholic fatty liver disease (NAFLD) is a condition that affects about 24% of people worldwide. Increased liver fat, inflammation, and, in the most severe cases, cell death are all characteristics of NAFLD. However, NAFLD pathogenesis and therapy are still not clear enough. Thus, this study aime...

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Autores principales: Aziz, Marina, Hemeda, Shabaan A., Albadrani, Ghadeer M., Fadl, Sabreen E., Elgendey, Fatma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113232/
https://www.ncbi.nlm.nih.gov/pubmed/37072469
http://dx.doi.org/10.1038/s41598-023-32584-7
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author Aziz, Marina
Hemeda, Shabaan A.
Albadrani, Ghadeer M.
Fadl, Sabreen E.
Elgendey, Fatma
author_facet Aziz, Marina
Hemeda, Shabaan A.
Albadrani, Ghadeer M.
Fadl, Sabreen E.
Elgendey, Fatma
author_sort Aziz, Marina
collection PubMed
description Nonalcoholic fatty liver disease (NAFLD) is a condition that affects about 24% of people worldwide. Increased liver fat, inflammation, and, in the most severe cases, cell death are all characteristics of NAFLD. However, NAFLD pathogenesis and therapy are still not clear enough. Thus, this study aimed to determine the effect of a high-cholesterol diet (HCD) inducing NAFLD on lipolytic gene expression, liver function, lipid profile, and antioxidant enzymes in rabbits and the modulatory effects of probiotic Lactobacillus acidophilus (L. acidophilus) on it. A total of 45 male New Zealand white rabbits, eight weeks old, were randomly divided into three groups of three replicates (5 rabbits/replicate). Rabbits in group I were given a basal diet; rabbits in group II were given a high-cholesterol diet that caused NAFLD; and rabbits in group III were given a high-cholesterol diet as well as probiotics in water for 8 weeks. The results showed that a high-cholesterol diet caused hepatic vacuolation and upregulated the genes for lipoprotein lipase (LPL), hepatic lipase (HL), and cholesteryl ester transfer protein (CETP). Downregulated low-density lipoprotein receptor (LDLr) gene, increased liver enzymes [alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH)], cholesterol, triglycerides (TG), low-density lipoprotein (LDL), glucose, and total bilirubin. On the other hand, it decreased high-density lipoprotein (HDL), total protein, albumin, and liver antioxidants [glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD)]. Supplementing with probiotics helped to return all parameters to normal levels. In conclusion, probiotic supplementation, especially L. acidophilus, protected against NAFLD, and restored lipolytic gene expression, liver functions, and antioxidants to normal levels.
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spelling pubmed-101132322023-04-20 Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits Aziz, Marina Hemeda, Shabaan A. Albadrani, Ghadeer M. Fadl, Sabreen E. Elgendey, Fatma Sci Rep Article Nonalcoholic fatty liver disease (NAFLD) is a condition that affects about 24% of people worldwide. Increased liver fat, inflammation, and, in the most severe cases, cell death are all characteristics of NAFLD. However, NAFLD pathogenesis and therapy are still not clear enough. Thus, this study aimed to determine the effect of a high-cholesterol diet (HCD) inducing NAFLD on lipolytic gene expression, liver function, lipid profile, and antioxidant enzymes in rabbits and the modulatory effects of probiotic Lactobacillus acidophilus (L. acidophilus) on it. A total of 45 male New Zealand white rabbits, eight weeks old, were randomly divided into three groups of three replicates (5 rabbits/replicate). Rabbits in group I were given a basal diet; rabbits in group II were given a high-cholesterol diet that caused NAFLD; and rabbits in group III were given a high-cholesterol diet as well as probiotics in water for 8 weeks. The results showed that a high-cholesterol diet caused hepatic vacuolation and upregulated the genes for lipoprotein lipase (LPL), hepatic lipase (HL), and cholesteryl ester transfer protein (CETP). Downregulated low-density lipoprotein receptor (LDLr) gene, increased liver enzymes [alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH)], cholesterol, triglycerides (TG), low-density lipoprotein (LDL), glucose, and total bilirubin. On the other hand, it decreased high-density lipoprotein (HDL), total protein, albumin, and liver antioxidants [glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD)]. Supplementing with probiotics helped to return all parameters to normal levels. In conclusion, probiotic supplementation, especially L. acidophilus, protected against NAFLD, and restored lipolytic gene expression, liver functions, and antioxidants to normal levels. Nature Publishing Group UK 2023-04-18 /pmc/articles/PMC10113232/ /pubmed/37072469 http://dx.doi.org/10.1038/s41598-023-32584-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Aziz, Marina
Hemeda, Shabaan A.
Albadrani, Ghadeer M.
Fadl, Sabreen E.
Elgendey, Fatma
Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title_full Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title_fullStr Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title_full_unstemmed Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title_short Ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
title_sort ameliorating effect of probiotic on nonalcoholic fatty liver disease and lipolytic gene expression in rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113232/
https://www.ncbi.nlm.nih.gov/pubmed/37072469
http://dx.doi.org/10.1038/s41598-023-32584-7
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