Cargando…
LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p
BACKGROUND: Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated. OBJECTIVE: We attempt to explain the mechanism of PVT1’s role in breast can...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113361/ https://www.ncbi.nlm.nih.gov/pubmed/36941464 http://dx.doi.org/10.1007/s13258-023-01368-8 |
_version_ | 1785027823173369856 |
---|---|
author | Qu, Huan Li, Xingxing Chen, Fei Zhang, Min Lu, Xun Gu, Yun Lv, Mingming Lu, Cheng |
author_facet | Qu, Huan Li, Xingxing Chen, Fei Zhang, Min Lu, Xun Gu, Yun Lv, Mingming Lu, Cheng |
author_sort | Qu, Huan |
collection | PubMed |
description | BACKGROUND: Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated. OBJECTIVE: We attempt to explain the mechanism of PVT1’s role in breast cancer from different perspectives. METHODS: We analyzed the expression of PVT1 and its correlation with the breast cancer related clinical data in the The Cancer Genome Atlas (TCGA) database. We used PVT1 overexpression and knockdown lentivirus to infect breast cancer MDA-MB-231 cell line for cell function verification, in vitro using CCK-8 to measure proliferation, flow cytometry to measure apoptosis, transwell test to measure invasion and migration ability, detecting cell extracellular acidification rate (ECAR) to assess glycolysis metabolism and explore the biological functions of PVT1 in breast cancer cells. Transcriptome sequencing was used to analyze the changes of related genes in cells after overexpression of PVT1. In vivo we used a xenograft model to study the effect of PVT1 on breast cancer. RESULTS: PVT1 was up-regulated in breast cancer tissues and was positively correlated with the clinical stage of breast cancer patients. Overexpression of PVT1 in vitro promoted cell proliferation, migration and invasion, and promoted tumor growth in vivo. Knockdown of PVT1 led to the opposite biological consequence. Further bioinformatics analysis showed that PVT1 changes the glycolysis metabolism of tumors through regulation of glycolysis-related genes. In addition, the expression of miR-145-5p is negatively correlated with PVT1. We consider the possibility of PVT1 promoting cell proliferation and metastasis by regulating the aerobic glucose metabolism in breast cancer cells through sponging the miR-145-5p. CONCLUSION: Our results reveal a potential pathway for competing endogenous RNA to regulate breast cancer glucose metabolism. PVT1 regulates glycolysis related genes expression by competitively binding to endogenous miR-145-5p in breast cancer cells to change the metabolic phenotype. This may Provide new ideas for precise molecular therapy targets for breast cancer. |
format | Online Article Text |
id | pubmed-10113361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-101133612023-04-20 LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p Qu, Huan Li, Xingxing Chen, Fei Zhang, Min Lu, Xun Gu, Yun Lv, Mingming Lu, Cheng Genes Genomics Research Article BACKGROUND: Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated. OBJECTIVE: We attempt to explain the mechanism of PVT1’s role in breast cancer from different perspectives. METHODS: We analyzed the expression of PVT1 and its correlation with the breast cancer related clinical data in the The Cancer Genome Atlas (TCGA) database. We used PVT1 overexpression and knockdown lentivirus to infect breast cancer MDA-MB-231 cell line for cell function verification, in vitro using CCK-8 to measure proliferation, flow cytometry to measure apoptosis, transwell test to measure invasion and migration ability, detecting cell extracellular acidification rate (ECAR) to assess glycolysis metabolism and explore the biological functions of PVT1 in breast cancer cells. Transcriptome sequencing was used to analyze the changes of related genes in cells after overexpression of PVT1. In vivo we used a xenograft model to study the effect of PVT1 on breast cancer. RESULTS: PVT1 was up-regulated in breast cancer tissues and was positively correlated with the clinical stage of breast cancer patients. Overexpression of PVT1 in vitro promoted cell proliferation, migration and invasion, and promoted tumor growth in vivo. Knockdown of PVT1 led to the opposite biological consequence. Further bioinformatics analysis showed that PVT1 changes the glycolysis metabolism of tumors through regulation of glycolysis-related genes. In addition, the expression of miR-145-5p is negatively correlated with PVT1. We consider the possibility of PVT1 promoting cell proliferation and metastasis by regulating the aerobic glucose metabolism in breast cancer cells through sponging the miR-145-5p. CONCLUSION: Our results reveal a potential pathway for competing endogenous RNA to regulate breast cancer glucose metabolism. PVT1 regulates glycolysis related genes expression by competitively binding to endogenous miR-145-5p in breast cancer cells to change the metabolic phenotype. This may Provide new ideas for precise molecular therapy targets for breast cancer. Springer Nature Singapore 2023-03-20 2023 /pmc/articles/PMC10113361/ /pubmed/36941464 http://dx.doi.org/10.1007/s13258-023-01368-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Qu, Huan Li, Xingxing Chen, Fei Zhang, Min Lu, Xun Gu, Yun Lv, Mingming Lu, Cheng LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title | LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title_full | LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title_fullStr | LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title_full_unstemmed | LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title_short | LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p |
title_sort | lncrna pvt1 influences breast cancer cells glycolysis through sponging mir-145-5p |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113361/ https://www.ncbi.nlm.nih.gov/pubmed/36941464 http://dx.doi.org/10.1007/s13258-023-01368-8 |
work_keys_str_mv | AT quhuan lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT lixingxing lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT chenfei lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT zhangmin lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT luxun lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT guyun lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT lvmingming lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p AT lucheng lncrnapvt1influencesbreastcancercellsglycolysisthroughspongingmir1455p |