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Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy

The objective of this study was to investigate the possible association between the single-nucleotide polymorphism, rs35569394, of the vascular endothelial growth factor (VEGF) gene and the risk of esophageal cancer (EC) in the Han Chinese population. A total of 290 EC subjects and 322 ethnically ma...

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Autores principales: Wang, Zishan, Li, Chenwei, Li, Xinjian, Shi, Jianguang, Wu, Weijie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113952/
https://www.ncbi.nlm.nih.gov/pubmed/34157252
http://dx.doi.org/10.17305/bjbms.2021.5891
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author Wang, Zishan
Li, Chenwei
Li, Xinjian
Shi, Jianguang
Wu, Weijie
author_facet Wang, Zishan
Li, Chenwei
Li, Xinjian
Shi, Jianguang
Wu, Weijie
author_sort Wang, Zishan
collection PubMed
description The objective of this study was to investigate the possible association between the single-nucleotide polymorphism, rs35569394, of the vascular endothelial growth factor (VEGF) gene and the risk of esophageal cancer (EC) in the Han Chinese population. A total of 290 EC subjects and 322 ethnically matched unrelated healthy controls free from the esophageal disease were studied. Genomic DNA was isolated from peripheral blood by salting out. Genotyping of VEGF rs35569394 polymorphism was carried out through polymerase chain reaction followed by agarose gel electrophoresis. The results showed that the distribution of genotypes was significantly different across the gender groups (p ═ 0.032) and clinical stages of the esophageal cancer (p ═ 0.034). VEGF rs35569394 was associated with EC risk (p ═ 0.012, OR ═ 1.34). A gender analysis breakdown showed that rs35569394-D allele frequency was significantly higher in females than in the controls (p ═ 0.0004, OR ═ 1.81). Moreover, significant associations were also found in females under the dominant model (II vs. ID+DD: χ(2) ═ 8.18, p ═ 0.003, OR ═ 2.12) and the recessive model (II+ID vs. DD: χ(2) ═ 8.25, p ═ 0.004, OR ═ 2.39). In addition, we found that the genotype, rs35569394-DD, was associated with a complete response and partial response to chemotherapy when compared with rs35569394-II (χ(2) ═ 4.67, p ═ 0.030, OR ═ 0.47). In conclusion, our case–control study showed that the VEGF rs35569394 was significantly associated with the clinical stages and the increased risk of EC in Han Chinese females. In addition, the genotype rs35569394-DD showed a better response to chemotherapy.
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spelling pubmed-101139522023-04-20 Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy Wang, Zishan Li, Chenwei Li, Xinjian Shi, Jianguang Wu, Weijie Biomol Biomed Research Article The objective of this study was to investigate the possible association between the single-nucleotide polymorphism, rs35569394, of the vascular endothelial growth factor (VEGF) gene and the risk of esophageal cancer (EC) in the Han Chinese population. A total of 290 EC subjects and 322 ethnically matched unrelated healthy controls free from the esophageal disease were studied. Genomic DNA was isolated from peripheral blood by salting out. Genotyping of VEGF rs35569394 polymorphism was carried out through polymerase chain reaction followed by agarose gel electrophoresis. The results showed that the distribution of genotypes was significantly different across the gender groups (p ═ 0.032) and clinical stages of the esophageal cancer (p ═ 0.034). VEGF rs35569394 was associated with EC risk (p ═ 0.012, OR ═ 1.34). A gender analysis breakdown showed that rs35569394-D allele frequency was significantly higher in females than in the controls (p ═ 0.0004, OR ═ 1.81). Moreover, significant associations were also found in females under the dominant model (II vs. ID+DD: χ(2) ═ 8.18, p ═ 0.003, OR ═ 2.12) and the recessive model (II+ID vs. DD: χ(2) ═ 8.25, p ═ 0.004, OR ═ 2.39). In addition, we found that the genotype, rs35569394-DD, was associated with a complete response and partial response to chemotherapy when compared with rs35569394-II (χ(2) ═ 4.67, p ═ 0.030, OR ═ 0.47). In conclusion, our case–control study showed that the VEGF rs35569394 was significantly associated with the clinical stages and the increased risk of EC in Han Chinese females. In addition, the genotype rs35569394-DD showed a better response to chemotherapy. Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2023-04-01 2023-03-16 /pmc/articles/PMC10113952/ /pubmed/34157252 http://dx.doi.org/10.17305/bjbms.2021.5891 Text en © 2021 Wang et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Wang, Zishan
Li, Chenwei
Li, Xinjian
Shi, Jianguang
Wu, Weijie
Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title_full Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title_fullStr Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title_full_unstemmed Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title_short Effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
title_sort effect of vascular endothelial growth factor rs35569394 in esophageal cancer and response to chemotherapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113952/
https://www.ncbi.nlm.nih.gov/pubmed/34157252
http://dx.doi.org/10.17305/bjbms.2021.5891
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