Massively parallel characterization of CRISPR activator efficacy in human induced pluripotent stem cells and neurons

CRISPR activation (CRISPRa) is an important tool to perturb transcription, but its effectiveness varies between target genes. We employ human pluripotent stem cells with thousands of randomly integrated barcoded reporters to assess epigenetic features that influence CRISPRa efficacy. Basal expressio...

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Detalles Bibliográficos
Autores principales: Wu, Qianxin, Wu, Junjing, Karim, Kaiser, Chen, Xi, Wang, Tengyao, Iwama, Sho, Carobbio, Stefania, Keen, Peter, Vidal-Puig, Antonio, Kotter, Mark R., Bassett, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10114495/
https://www.ncbi.nlm.nih.gov/pubmed/36917981
http://dx.doi.org/10.1016/j.molcel.2023.02.011

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