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Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition

Candida albicans can cause two major types of infections: superficial infection and systemic candidiasis. C. albicans infects diverse host niches, owing to a wide range of virulence factors and attributes, such as morphological transitions and phenotypic switching. C. albicans uses glycolysis, follo...

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Autores principales: Okabayashi, Ken, Ogawa, Hitomi, Hirai, Yuto, Nagata, Kureha, Sato, Yukiko, Narita, Takanori, Satoh, Kazuo, Makimura, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10115278/
https://www.ncbi.nlm.nih.gov/pubmed/37075046
http://dx.doi.org/10.1371/journal.pone.0284353
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author Okabayashi, Ken
Ogawa, Hitomi
Hirai, Yuto
Nagata, Kureha
Sato, Yukiko
Narita, Takanori
Satoh, Kazuo
Makimura, Koichi
author_facet Okabayashi, Ken
Ogawa, Hitomi
Hirai, Yuto
Nagata, Kureha
Sato, Yukiko
Narita, Takanori
Satoh, Kazuo
Makimura, Koichi
author_sort Okabayashi, Ken
collection PubMed
description Candida albicans can cause two major types of infections: superficial infection and systemic candidiasis. C. albicans infects diverse host niches, owing to a wide range of virulence factors and attributes, such as morphological transitions and phenotypic switching. C. albicans uses glycolysis, followed by alcoholic fermentation or mitochondrial respiration to rapidly generate ATP under aerobic conditions. In this study, we quantified the mRNA expression of several glycolysis-related enzymes associated with the initial phase of environmental changes using two strains: a type strain, NBRC 1385, and a strain from a patient with auto-brewery syndrome, LSEM 550. Additionally, we analyzed the regulation of a rate-limiting enzyme in glycolysis, phosphofructokinase 1 (PFK1). Our results showed that the mRNA expression of enzymes in the middle and last stages of glycolysis and alcoholic fermentation increased, and that of mitochondrial respiration enzymes decreased under short-term anaerobic conditions. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) administration showed similar results under anaerobic conditions. Moreover, PFK1 maintained its regulatory effect under different conditions; no significant change was observed in its mRNA expression. Our results suggest that C. albicans obtains energy via carbohydrate catabolism in the early phase of environmental change and survives in various parts of the host.
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spelling pubmed-101152782023-04-20 Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition Okabayashi, Ken Ogawa, Hitomi Hirai, Yuto Nagata, Kureha Sato, Yukiko Narita, Takanori Satoh, Kazuo Makimura, Koichi PLoS One Research Article Candida albicans can cause two major types of infections: superficial infection and systemic candidiasis. C. albicans infects diverse host niches, owing to a wide range of virulence factors and attributes, such as morphological transitions and phenotypic switching. C. albicans uses glycolysis, followed by alcoholic fermentation or mitochondrial respiration to rapidly generate ATP under aerobic conditions. In this study, we quantified the mRNA expression of several glycolysis-related enzymes associated with the initial phase of environmental changes using two strains: a type strain, NBRC 1385, and a strain from a patient with auto-brewery syndrome, LSEM 550. Additionally, we analyzed the regulation of a rate-limiting enzyme in glycolysis, phosphofructokinase 1 (PFK1). Our results showed that the mRNA expression of enzymes in the middle and last stages of glycolysis and alcoholic fermentation increased, and that of mitochondrial respiration enzymes decreased under short-term anaerobic conditions. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) administration showed similar results under anaerobic conditions. Moreover, PFK1 maintained its regulatory effect under different conditions; no significant change was observed in its mRNA expression. Our results suggest that C. albicans obtains energy via carbohydrate catabolism in the early phase of environmental change and survives in various parts of the host. Public Library of Science 2023-04-19 /pmc/articles/PMC10115278/ /pubmed/37075046 http://dx.doi.org/10.1371/journal.pone.0284353 Text en © 2023 Okabayashi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Okabayashi, Ken
Ogawa, Hitomi
Hirai, Yuto
Nagata, Kureha
Sato, Yukiko
Narita, Takanori
Satoh, Kazuo
Makimura, Koichi
Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title_full Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title_fullStr Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title_full_unstemmed Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title_short Changes in the mRNA expression of glycolysis-related enzymes of Candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
title_sort changes in the mrna expression of glycolysis-related enzymes of candida albicans during inhibition of intramitochondrial catabolism under anaerobic condition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10115278/
https://www.ncbi.nlm.nih.gov/pubmed/37075046
http://dx.doi.org/10.1371/journal.pone.0284353
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