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Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma

Leucocyte immunoglobulin‐like receptors (LILRs) are closely related to tumourigenesis, but their clinical value in early‐stage pancreatic ductal adenocarcinoma (PDAC) after pancreaticoduodenectomy remains unknown. Kaplan–Meier and Cox proportional hazards regression models is used to investigate the...

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Autores principales: Gao, Qiang, Mo, Shutian, Han, Chuangye, Liao, Xiwen, Yang, Chengkun, Wang, Xiangkun, Liang, Tianyi, He, Yongfei, Chen, Zijun, Zhu, Guangzhi, Su, Hao, Ye, Xinping, Peng, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116025/
https://www.ncbi.nlm.nih.gov/pubmed/36748687
http://dx.doi.org/10.1049/syb2.12058
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author Gao, Qiang
Mo, Shutian
Han, Chuangye
Liao, Xiwen
Yang, Chengkun
Wang, Xiangkun
Liang, Tianyi
He, Yongfei
Chen, Zijun
Zhu, Guangzhi
Su, Hao
Ye, Xinping
Peng, Tao
author_facet Gao, Qiang
Mo, Shutian
Han, Chuangye
Liao, Xiwen
Yang, Chengkun
Wang, Xiangkun
Liang, Tianyi
He, Yongfei
Chen, Zijun
Zhu, Guangzhi
Su, Hao
Ye, Xinping
Peng, Tao
author_sort Gao, Qiang
collection PubMed
description Leucocyte immunoglobulin‐like receptors (LILRs) are closely related to tumourigenesis, but their clinical value in early‐stage pancreatic ductal adenocarcinoma (PDAC) after pancreaticoduodenectomy remains unknown. Kaplan–Meier and Cox proportional hazards regression models is used to investigate the association between LILR expression and prognosis in tumour biopsies and peripheral blood mononuclear cells. Risk score was calculated for each patient based on the prognostic model. DAVID, STRING, GeneMANIA, and GSEA were used to conduct pathway and functional analyses. The CIBERSORT algorithm is used to analyse tumour‐infiltrating immune cells. Survival analysis showed that high levels of LILRA4 (p = 0.006) and LILRB4 (p = 0.04) were significantly associated with better overall survival. High levels of LILRA2 (p = 0.008) and LILRB4 (p = 0.038) were significantly associated with better relapse‐free survival. JAK‐STAT signalling pathway, regulation of T cell activation, regulation of the immune effector process, and tumour necrosis factor superfamily cytokine production were involved in molecular mechanisms that affected poor prognoses in the high‐risk group in GSEA. CIBERSORT demonstrated that the high‐risk group had significantly higher infiltrating fraction of memory‐activated CD4 T cells and activated NK cells and lower fraction of resting dendritic cells and neutrophils. LILRB4 plays crucial roles in affecting the clinical outcomes of early‐stage PDAC.
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spelling pubmed-101160252023-04-21 Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma Gao, Qiang Mo, Shutian Han, Chuangye Liao, Xiwen Yang, Chengkun Wang, Xiangkun Liang, Tianyi He, Yongfei Chen, Zijun Zhu, Guangzhi Su, Hao Ye, Xinping Peng, Tao IET Syst Biol Original Research Leucocyte immunoglobulin‐like receptors (LILRs) are closely related to tumourigenesis, but their clinical value in early‐stage pancreatic ductal adenocarcinoma (PDAC) after pancreaticoduodenectomy remains unknown. Kaplan–Meier and Cox proportional hazards regression models is used to investigate the association between LILR expression and prognosis in tumour biopsies and peripheral blood mononuclear cells. Risk score was calculated for each patient based on the prognostic model. DAVID, STRING, GeneMANIA, and GSEA were used to conduct pathway and functional analyses. The CIBERSORT algorithm is used to analyse tumour‐infiltrating immune cells. Survival analysis showed that high levels of LILRA4 (p = 0.006) and LILRB4 (p = 0.04) were significantly associated with better overall survival. High levels of LILRA2 (p = 0.008) and LILRB4 (p = 0.038) were significantly associated with better relapse‐free survival. JAK‐STAT signalling pathway, regulation of T cell activation, regulation of the immune effector process, and tumour necrosis factor superfamily cytokine production were involved in molecular mechanisms that affected poor prognoses in the high‐risk group in GSEA. CIBERSORT demonstrated that the high‐risk group had significantly higher infiltrating fraction of memory‐activated CD4 T cells and activated NK cells and lower fraction of resting dendritic cells and neutrophils. LILRB4 plays crucial roles in affecting the clinical outcomes of early‐stage PDAC. John Wiley and Sons Inc. 2023-02-07 /pmc/articles/PMC10116025/ /pubmed/36748687 http://dx.doi.org/10.1049/syb2.12058 Text en © 2023 The Authors. IET Systems Biology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Gao, Qiang
Mo, Shutian
Han, Chuangye
Liao, Xiwen
Yang, Chengkun
Wang, Xiangkun
Liang, Tianyi
He, Yongfei
Chen, Zijun
Zhu, Guangzhi
Su, Hao
Ye, Xinping
Peng, Tao
Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title_full Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title_fullStr Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title_full_unstemmed Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title_short Comprehensive analysis of LILR family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
title_sort comprehensive analysis of lilr family genes expression and tumour‐infiltrating immune cells in early‐stage pancreatic ductal adenocarcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116025/
https://www.ncbi.nlm.nih.gov/pubmed/36748687
http://dx.doi.org/10.1049/syb2.12058
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