The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan

Ceramide glucosyltransferase (CGT) adds sugar moieties to ceramide, forming glucosylceramides that play roles in immune signaling, stress response, and host-bacterial interactions. Here, we examined whether mutations in cgt block the beneficial effects of Bacillus subtilis on C. elegans lifespan. We...

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Autores principales: Arvin, Chelsey L, Sibila, Zachary, Lamendella, Regina, Chan, Jason, Staab, Trisha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116348/
https://www.ncbi.nlm.nih.gov/pubmed/37090153
http://dx.doi.org/10.17912/micropub.biology.000758
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author Arvin, Chelsey L
Sibila, Zachary
Lamendella, Regina
Chan, Jason
Staab, Trisha
author_facet Arvin, Chelsey L
Sibila, Zachary
Lamendella, Regina
Chan, Jason
Staab, Trisha
author_sort Arvin, Chelsey L
collection PubMed
description Ceramide glucosyltransferase (CGT) adds sugar moieties to ceramide, forming glucosylceramides that play roles in immune signaling, stress response, and host-bacterial interactions. Here, we examined whether mutations in cgt block the beneficial effects of Bacillus subtilis on C. elegans lifespan. We found that loss of cgt-1 or cgt-3 reduces lifespan compared to wildtype worms, but did not prevent the lifespan-extending phenotype of B. subtilis . However, cgt-1(ok1045) and cgt-3(tm504) did play a minor role in blocking stress resistance of 5-day old worms treated with B. subtilis . Further studying CGTs may elucidate potential roles of glucosylceramides in host-bacterial interaction.
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spelling pubmed-101163482023-04-21 The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan Arvin, Chelsey L Sibila, Zachary Lamendella, Regina Chan, Jason Staab, Trisha MicroPubl Biol New Finding Ceramide glucosyltransferase (CGT) adds sugar moieties to ceramide, forming glucosylceramides that play roles in immune signaling, stress response, and host-bacterial interactions. Here, we examined whether mutations in cgt block the beneficial effects of Bacillus subtilis on C. elegans lifespan. We found that loss of cgt-1 or cgt-3 reduces lifespan compared to wildtype worms, but did not prevent the lifespan-extending phenotype of B. subtilis . However, cgt-1(ok1045) and cgt-3(tm504) did play a minor role in blocking stress resistance of 5-day old worms treated with B. subtilis . Further studying CGTs may elucidate potential roles of glucosylceramides in host-bacterial interaction. Caltech Library 2023-04-05 /pmc/articles/PMC10116348/ /pubmed/37090153 http://dx.doi.org/10.17912/micropub.biology.000758 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Arvin, Chelsey L
Sibila, Zachary
Lamendella, Regina
Chan, Jason
Staab, Trisha
The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title_full The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title_fullStr The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title_full_unstemmed The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title_short The impact of C. elegans ceramide glucosyltransferase enzymes on the beneficial effects of B. subtilis lifespan
title_sort impact of c. elegans ceramide glucosyltransferase enzymes on the beneficial effects of b. subtilis lifespan
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116348/
https://www.ncbi.nlm.nih.gov/pubmed/37090153
http://dx.doi.org/10.17912/micropub.biology.000758
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