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Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography

We present membrane-based steric exclusion chromatography (SXC) as a universal capture step for purification of adeno-associated virus (AAV) gene transfer vectors independent of their serotype and surface characteristics. SXC is performed by mixing an unpurified cell culture supernatant containing A...

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Detalles Bibliográficos
Autores principales:	Marichal-Gallardo, Pavel, Börner, Kathleen, Pieler, Michael M., Sonntag-Buck, Vera, Obr, Martin, Bejarano, David, Wolff, Michael W., Kräusslich, Hans-Georg, Reichl, Udo, Grimm, Dirk
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2021
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116406/ https://www.ncbi.nlm.nih.gov/pubmed/33554722 http://dx.doi.org/10.1089/hum.2019.284

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