Cargando…

Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress

Hyperosmolality-gated calcium-permeable channels (OSCA) are Ca(2 +) nonselective cation channels that contain the calcium-dependent DUF221 domain, which plays an important role in plant response to stress and growth. However, the OSCA gene has not been fully identified and analyzed in sunflowers. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Shan, Feibiao, Wu, Yue, Du, Ruixia, Yang, Qinfang, Liu, Chunhui, Wang, Yongxing, Zhang, Chun, Chen, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117387/
https://www.ncbi.nlm.nih.gov/pubmed/37090105
http://dx.doi.org/10.7717/peerj.15089
_version_ 1785028601712738304
author Shan, Feibiao
Wu, Yue
Du, Ruixia
Yang, Qinfang
Liu, Chunhui
Wang, Yongxing
Zhang, Chun
Chen, Yang
author_facet Shan, Feibiao
Wu, Yue
Du, Ruixia
Yang, Qinfang
Liu, Chunhui
Wang, Yongxing
Zhang, Chun
Chen, Yang
author_sort Shan, Feibiao
collection PubMed
description Hyperosmolality-gated calcium-permeable channels (OSCA) are Ca(2 +) nonselective cation channels that contain the calcium-dependent DUF221 domain, which plays an important role in plant response to stress and growth. However, the OSCA gene has not been fully identified and analyzed in sunflowers. In this study, we comprehensively analyzed the number, structure, collinearity, and phylogeny of the OSCA gene family in the sunflower, six Compositae species (Arctium lappa, Chrysanthemum morifolium, Cichorium endivia, Cichorium intybus, Lactuca sativa var. Angustata, and Carthamus tinctorius), and six other plants (soybean, Arabidopsis thaliana, rice, grape, and maize). The expression of the sunflower OSCA gene in nine different tissues, six different hormones, and NaCl stress conditions were analyzed based on transcriptome data and qRT–PCR. A total of 15 OSCA proteins, distributed on 10 chromosomes, were identified in the sunflower, and all of them were located in the endoplasmic reticulum. Using the phylogenetic tree, collinearity, gene structure, and motif analysis of the six Compositae species and six other plants, we found that the sunflower OSCA protein had only three subfamilies and lacked the Group 4 subfamily, which is conserved in the evolution of Compositae and subject to purification selection. The OSCA gene structure and motif analysis of the sunflower and six Compositae showed that there was a positive correlation between the number of motifs of most genes and the length of the gene, different subfamilies had different motifs, and the Group 4 subfamily had the smallest number of genes and the simplest gene structure. RNA-seq and qRT–PCR analysis showed that the expression levels of most OSCA genes in the sunflower changed to varying degrees under salt stress, and HaOSCA2.6 and HaOSCA3.1 were the most important in the sunflower’s response to salt stress. The coexpression network of the sunflower genes under salt stress was constructed based on weighted gene co-expression network analysis (WGCNA). In conclusion, our findings suggest that the OSCA gene family is conserved during the sunflower’s evolution and plays an important role in salt tolerance. These results will deepen our understanding of the evolutionary relationship of the sunflower OSCA gene family and provide a basis for their functional studies under salt stress.
format Online
Article
Text
id pubmed-10117387
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-101173872023-04-21 Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress Shan, Feibiao Wu, Yue Du, Ruixia Yang, Qinfang Liu, Chunhui Wang, Yongxing Zhang, Chun Chen, Yang PeerJ Agricultural Science Hyperosmolality-gated calcium-permeable channels (OSCA) are Ca(2 +) nonselective cation channels that contain the calcium-dependent DUF221 domain, which plays an important role in plant response to stress and growth. However, the OSCA gene has not been fully identified and analyzed in sunflowers. In this study, we comprehensively analyzed the number, structure, collinearity, and phylogeny of the OSCA gene family in the sunflower, six Compositae species (Arctium lappa, Chrysanthemum morifolium, Cichorium endivia, Cichorium intybus, Lactuca sativa var. Angustata, and Carthamus tinctorius), and six other plants (soybean, Arabidopsis thaliana, rice, grape, and maize). The expression of the sunflower OSCA gene in nine different tissues, six different hormones, and NaCl stress conditions were analyzed based on transcriptome data and qRT–PCR. A total of 15 OSCA proteins, distributed on 10 chromosomes, were identified in the sunflower, and all of them were located in the endoplasmic reticulum. Using the phylogenetic tree, collinearity, gene structure, and motif analysis of the six Compositae species and six other plants, we found that the sunflower OSCA protein had only three subfamilies and lacked the Group 4 subfamily, which is conserved in the evolution of Compositae and subject to purification selection. The OSCA gene structure and motif analysis of the sunflower and six Compositae showed that there was a positive correlation between the number of motifs of most genes and the length of the gene, different subfamilies had different motifs, and the Group 4 subfamily had the smallest number of genes and the simplest gene structure. RNA-seq and qRT–PCR analysis showed that the expression levels of most OSCA genes in the sunflower changed to varying degrees under salt stress, and HaOSCA2.6 and HaOSCA3.1 were the most important in the sunflower’s response to salt stress. The coexpression network of the sunflower genes under salt stress was constructed based on weighted gene co-expression network analysis (WGCNA). In conclusion, our findings suggest that the OSCA gene family is conserved during the sunflower’s evolution and plays an important role in salt tolerance. These results will deepen our understanding of the evolutionary relationship of the sunflower OSCA gene family and provide a basis for their functional studies under salt stress. PeerJ Inc. 2023-04-17 /pmc/articles/PMC10117387/ /pubmed/37090105 http://dx.doi.org/10.7717/peerj.15089 Text en ©2023 Shan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Shan, Feibiao
Wu, Yue
Du, Ruixia
Yang, Qinfang
Liu, Chunhui
Wang, Yongxing
Zhang, Chun
Chen, Yang
Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title_full Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title_fullStr Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title_full_unstemmed Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title_short Evolutionary analysis of the OSCA gene family in sunflower (Helianthus annuus L) and expression analysis under NaCl stress
title_sort evolutionary analysis of the osca gene family in sunflower (helianthus annuus l) and expression analysis under nacl stress
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117387/
https://www.ncbi.nlm.nih.gov/pubmed/37090105
http://dx.doi.org/10.7717/peerj.15089
work_keys_str_mv AT shanfeibiao evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT wuyue evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT duruixia evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT yangqinfang evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT liuchunhui evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT wangyongxing evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT zhangchun evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress
AT chenyang evolutionaryanalysisoftheoscagenefamilyinsunflowerhelianthusannuuslandexpressionanalysisundernaclstress