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MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer
PURPOSE: Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCa tumorigenesis. METHODS: The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 and EJ were used to transfect...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Makerere Medical School
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117455/ https://www.ncbi.nlm.nih.gov/pubmed/37092087 http://dx.doi.org/10.4314/ahs.v22i4.8 |
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author | Liang, Jun Feng Li, Pei hua Zhu, Yong Zheng, Shuai shuai Liu, Jing wei Song, Shi qiang |
author_facet | Liang, Jun Feng Li, Pei hua Zhu, Yong Zheng, Shuai shuai Liu, Jing wei Song, Shi qiang |
author_sort | Liang, Jun Feng |
collection | PubMed |
description | PURPOSE: Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCa tumorigenesis. METHODS: The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 and EJ were used to transfect miR-186 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-186 and ADAMTS12. MTT method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability was detected by transwell assay. The protein level of ADAMTS12, β-catenin, GSK-3β and p-GSK-3β was determined using western blot analysis. RESULTS: MiR-186 was negatively correlated with the expression of ADAMTS12 in BCa tissues. Further research confirmed that ADAMTS12 is the direct target of miR-186. In addition, overexpression of miR-186 down-regulated the expression of ADAMTS12, inhibiting cell viability and apoptosis, while knockout of miR-186 led to the opposite result. miR-186 also inhibits the phosphorylation of GSK-3 β and β-catenin without changing the total GSK-3β level. Our study shows that miR-186 has a negative regulatory effect on the expression of ADAMTS12 in clinical specimens and in vitro. miR-186 can inhibit the proliferation and invasion of BCa cells. CONCLUSIONS: miR-186 has the potential to be used as a biomarker in the early detection of BCa. |
format | Online Article Text |
id | pubmed-10117455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Makerere Medical School |
record_format | MEDLINE/PubMed |
spelling | pubmed-101174552023-04-21 MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer Liang, Jun Feng Li, Pei hua Zhu, Yong Zheng, Shuai shuai Liu, Jing wei Song, Shi qiang Afr Health Sci Articles PURPOSE: Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCa tumorigenesis. METHODS: The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 and EJ were used to transfect miR-186 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-186 and ADAMTS12. MTT method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability was detected by transwell assay. The protein level of ADAMTS12, β-catenin, GSK-3β and p-GSK-3β was determined using western blot analysis. RESULTS: MiR-186 was negatively correlated with the expression of ADAMTS12 in BCa tissues. Further research confirmed that ADAMTS12 is the direct target of miR-186. In addition, overexpression of miR-186 down-regulated the expression of ADAMTS12, inhibiting cell viability and apoptosis, while knockout of miR-186 led to the opposite result. miR-186 also inhibits the phosphorylation of GSK-3 β and β-catenin without changing the total GSK-3β level. Our study shows that miR-186 has a negative regulatory effect on the expression of ADAMTS12 in clinical specimens and in vitro. miR-186 can inhibit the proliferation and invasion of BCa cells. CONCLUSIONS: miR-186 has the potential to be used as a biomarker in the early detection of BCa. Makerere Medical School 2022-12 /pmc/articles/PMC10117455/ /pubmed/37092087 http://dx.doi.org/10.4314/ahs.v22i4.8 Text en © 2022 Liang JF et al. https://creativecommons.org/licenses/by/4.0/Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Liang, Jun Feng Li, Pei hua Zhu, Yong Zheng, Shuai shuai Liu, Jing wei Song, Shi qiang MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title | MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title_full | MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title_fullStr | MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title_full_unstemmed | MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title_short | MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer |
title_sort | microrna-186 suppresses cell proliferation and metastasis in bladder cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117455/ https://www.ncbi.nlm.nih.gov/pubmed/37092087 http://dx.doi.org/10.4314/ahs.v22i4.8 |
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