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Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods

The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine. Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity. In this stu...

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Autores principales: Huang, Yuanyuan, Xie, Wen, Tang, Ting, Chen, Huaguo, Zhou, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117789/
https://www.ncbi.nlm.nih.gov/pubmed/37090772
http://dx.doi.org/10.3389/fnut.2023.1125831
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author Huang, Yuanyuan
Xie, Wen
Tang, Ting
Chen, Huaguo
Zhou, Xin
author_facet Huang, Yuanyuan
Xie, Wen
Tang, Ting
Chen, Huaguo
Zhou, Xin
author_sort Huang, Yuanyuan
collection PubMed
description The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine. Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity. In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared. According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS. Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques. The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods. According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides. This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
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spelling pubmed-101177892023-04-21 Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods Huang, Yuanyuan Xie, Wen Tang, Ting Chen, Huaguo Zhou, Xin Front Nutr Nutrition The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine. Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity. In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared. According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS. Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques. The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods. According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides. This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity. Frontiers Media S.A. 2023-04-06 /pmc/articles/PMC10117789/ /pubmed/37090772 http://dx.doi.org/10.3389/fnut.2023.1125831 Text en Copyright © 2023 Huang, Xie, Tang, Chen and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Huang, Yuanyuan
Xie, Wen
Tang, Ting
Chen, Huaguo
Zhou, Xin
Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title_full Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title_fullStr Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title_full_unstemmed Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title_short Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods
title_sort structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from mori fructus based on different extraction methods
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117789/
https://www.ncbi.nlm.nih.gov/pubmed/37090772
http://dx.doi.org/10.3389/fnut.2023.1125831
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