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Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization

Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated virus...

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Autores principales: Birch, Julie Melsted, Leijon, Mikael, Nielsen, Søren Saxmose, Struve, Tina, Jensen, Henrik Elvang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117860/
https://www.ncbi.nlm.nih.gov/pubmed/37334236
http://dx.doi.org/10.1093/femsmc/xtab005
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author Birch, Julie Melsted
Leijon, Mikael
Nielsen, Søren Saxmose
Struve, Tina
Jensen, Henrik Elvang
author_facet Birch, Julie Melsted
Leijon, Mikael
Nielsen, Søren Saxmose
Struve, Tina
Jensen, Henrik Elvang
author_sort Birch, Julie Melsted
collection PubMed
description Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope(®) 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome.
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spelling pubmed-101178602023-06-16 Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization Birch, Julie Melsted Leijon, Mikael Nielsen, Søren Saxmose Struve, Tina Jensen, Henrik Elvang FEMS Microbes Research Article Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (Neovison vison). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen in situ hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope(®) 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome. Oxford University Press 2021-04-16 /pmc/articles/PMC10117860/ /pubmed/37334236 http://dx.doi.org/10.1093/femsmc/xtab005 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Birch, Julie Melsted
Leijon, Mikael
Nielsen, Søren Saxmose
Struve, Tina
Jensen, Henrik Elvang
Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title_full Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title_fullStr Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title_full_unstemmed Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title_short Visualization of intestinal infections with astro- and sapovirus in mink (Neovison vison) kits by in situ hybridization
title_sort visualization of intestinal infections with astro- and sapovirus in mink (neovison vison) kits by in situ hybridization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117860/
https://www.ncbi.nlm.nih.gov/pubmed/37334236
http://dx.doi.org/10.1093/femsmc/xtab005
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