Cargando…
Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
INTRODUCTION: The leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality. METHODS: To explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred l...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118011/ https://www.ncbi.nlm.nih.gov/pubmed/37089651 http://dx.doi.org/10.3389/fpls.2023.1183398 |
_version_ | 1785028715389911040 |
---|---|
author | Wang, Lixia Zhang, Shu Zhang, Ye Li, Jingjuan Zhang, Yihui Zhou, Dandan Li, Cheng He, Lilong Li, Huayin Wang, Fengde Gao, Jianwei |
author_facet | Wang, Lixia Zhang, Shu Zhang, Ye Li, Jingjuan Zhang, Yihui Zhou, Dandan Li, Cheng He, Lilong Li, Huayin Wang, Fengde Gao, Jianwei |
author_sort | Wang, Lixia |
collection | PubMed |
description | INTRODUCTION: The leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality. METHODS: To explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred lines of Chinese cabbage, Y2 (large leaf) and Y7 (small leaf). Furtherly, the transcriptome and cis-acting elements analyses were conducted. RESULTS AND DISCUSSION: According to our results, Y2 exhibited a higher growth rate than Y7 during the whole growth stage. In addition, the significant higher leaf number was observed in Y2 than in Y7. There was no significant difference in the number of epidermal cells and guard cells per square millimeter between Y2 and Y7 leaves. It indicated that cell numbers caused the difference in leaf size. The measurement of phytohormone content confirmed that GA1 and GA3 mainly play essential roles in the early stage of leaf growth, and IPA and ABA were in the whole leaf growth period in regulating the cell proliferation difference between Y2 and Y7. Transcriptome analysis revealed that cyclins BraA09g010980.3C (CYCB) and BraA10g027420.3C (CYCD) were mainly responsible for the leaf size difference between Y2 and Y7 Chinese cabbage. Further, we revealed that the transcription factors BraA09gMYB47 and BraA06gMYB88 played critical roles in the difference of leaf size between Y2 and Y7 through the regulation of cell proliferation. CONCLUSION: This observation not only offers essential insights into understanding the regulation mechanism of leaf development, also provides a promising breeding strategy to improve Chinese cabbage yield. |
format | Online Article Text |
id | pubmed-10118011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101180112023-04-21 Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) Wang, Lixia Zhang, Shu Zhang, Ye Li, Jingjuan Zhang, Yihui Zhou, Dandan Li, Cheng He, Lilong Li, Huayin Wang, Fengde Gao, Jianwei Front Plant Sci Plant Science INTRODUCTION: The leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality. METHODS: To explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred lines of Chinese cabbage, Y2 (large leaf) and Y7 (small leaf). Furtherly, the transcriptome and cis-acting elements analyses were conducted. RESULTS AND DISCUSSION: According to our results, Y2 exhibited a higher growth rate than Y7 during the whole growth stage. In addition, the significant higher leaf number was observed in Y2 than in Y7. There was no significant difference in the number of epidermal cells and guard cells per square millimeter between Y2 and Y7 leaves. It indicated that cell numbers caused the difference in leaf size. The measurement of phytohormone content confirmed that GA1 and GA3 mainly play essential roles in the early stage of leaf growth, and IPA and ABA were in the whole leaf growth period in regulating the cell proliferation difference between Y2 and Y7. Transcriptome analysis revealed that cyclins BraA09g010980.3C (CYCB) and BraA10g027420.3C (CYCD) were mainly responsible for the leaf size difference between Y2 and Y7 Chinese cabbage. Further, we revealed that the transcription factors BraA09gMYB47 and BraA06gMYB88 played critical roles in the difference of leaf size between Y2 and Y7 through the regulation of cell proliferation. CONCLUSION: This observation not only offers essential insights into understanding the regulation mechanism of leaf development, also provides a promising breeding strategy to improve Chinese cabbage yield. Frontiers Media S.A. 2023-04-06 /pmc/articles/PMC10118011/ /pubmed/37089651 http://dx.doi.org/10.3389/fpls.2023.1183398 Text en Copyright © 2023 Wang, Zhang, Zhang, Li, Zhang, Zhou, Li, He, Li, Wang and Gao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Lixia Zhang, Shu Zhang, Ye Li, Jingjuan Zhang, Yihui Zhou, Dandan Li, Cheng He, Lilong Li, Huayin Wang, Fengde Gao, Jianwei Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title_full | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title_fullStr | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title_full_unstemmed | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title_short | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
title_sort | integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in chinese cabbage (brassica rapa l. ssp. pekinensis) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118011/ https://www.ncbi.nlm.nih.gov/pubmed/37089651 http://dx.doi.org/10.3389/fpls.2023.1183398 |
work_keys_str_mv | AT wanglixia integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT zhangshu integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT zhangye integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT lijingjuan integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT zhangyihui integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT zhoudandan integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT licheng integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT helilong integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT lihuayin integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT wangfengde integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis AT gaojianwei integrativeanalysisofphysiologybiochemistryandtranscriptomerevealsthemechanismofleafsizeformationinchinesecabbagebrassicarapalssppekinensis |