Cargando…
Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs
OBJECTIVE: To assess the effects of enfuvirtide on pregnancy in albino rats and their fetuses. METHODS: Forty pregnant EPM 1 Wistar rats were randomly allocated into four groups: control (E) (distilled water twice/day), G1 (4mg/kg/day enfuvirtide), G2 (12mg/kg/day enfuvirtide), and G3 (36mg/kg/day e...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Israelita de Ensino e Pesquisa Albert Einstein
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118372/ https://www.ncbi.nlm.nih.gov/pubmed/37075461 http://dx.doi.org/10.31744/einstein_journal/2023AO0230 |
_version_ | 1785028794075054080 |
---|---|
author | de Moraes, Renata Delphim Araujo, Edward Castelo, Adauto Peixoto, Alberto Borges Amed, Abês Mahmed |
author_facet | de Moraes, Renata Delphim Araujo, Edward Castelo, Adauto Peixoto, Alberto Borges Amed, Abês Mahmed |
author_sort | de Moraes, Renata Delphim |
collection | PubMed |
description | OBJECTIVE: To assess the effects of enfuvirtide on pregnancy in albino rats and their fetuses. METHODS: Forty pregnant EPM 1 Wistar rats were randomly allocated into four groups: control (E) (distilled water twice/day), G1 (4mg/kg/day enfuvirtide), G2 (12mg/kg/day enfuvirtide), and G3 (36mg/kg/day enfuvirtide) groups. On the 20th day of gestation, the rats were anesthetized and subjected to cesarean section. Their blood was collected for laboratory analysis, and they were sacrificed. The offspring’s fragments of their kidneys, liver, and placentas and the maternal rats’ fragments of their lungs, kidneys, and liver were separated in the immediate postpartum period for light microscopy analysis. RESULTS: No maternal deaths occurred. In the second week at the end of pregnancy, the mean weight of the G3 Group was significantly lower than that of the G2 Group (p=0.029 and p=0.028, respectively). Analyzing blood laboratory parameters, the G1 Group had the lowest mean amylase level, and the G2 Group had the lowest mean hemoglobin level and the highest mean platelet count. In the morphological analysis, there were no changes in organs, such as the kidneys and liver, in both the maternal rats and offspring. Three maternal rats in the G3 Group had pulmonary inflammation in the lungs. CONCLUSION: Enfuvirtide has no significant adverse effects on pregnancy, conceptual products, or functional alterations in maternal rats. |
format | Online Article Text |
id | pubmed-10118372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
record_format | MEDLINE/PubMed |
spelling | pubmed-101183722023-04-21 Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs de Moraes, Renata Delphim Araujo, Edward Castelo, Adauto Peixoto, Alberto Borges Amed, Abês Mahmed Einstein (Sao Paulo) Original Article OBJECTIVE: To assess the effects of enfuvirtide on pregnancy in albino rats and their fetuses. METHODS: Forty pregnant EPM 1 Wistar rats were randomly allocated into four groups: control (E) (distilled water twice/day), G1 (4mg/kg/day enfuvirtide), G2 (12mg/kg/day enfuvirtide), and G3 (36mg/kg/day enfuvirtide) groups. On the 20th day of gestation, the rats were anesthetized and subjected to cesarean section. Their blood was collected for laboratory analysis, and they were sacrificed. The offspring’s fragments of their kidneys, liver, and placentas and the maternal rats’ fragments of their lungs, kidneys, and liver were separated in the immediate postpartum period for light microscopy analysis. RESULTS: No maternal deaths occurred. In the second week at the end of pregnancy, the mean weight of the G3 Group was significantly lower than that of the G2 Group (p=0.029 and p=0.028, respectively). Analyzing blood laboratory parameters, the G1 Group had the lowest mean amylase level, and the G2 Group had the lowest mean hemoglobin level and the highest mean platelet count. In the morphological analysis, there were no changes in organs, such as the kidneys and liver, in both the maternal rats and offspring. Three maternal rats in the G3 Group had pulmonary inflammation in the lungs. CONCLUSION: Enfuvirtide has no significant adverse effects on pregnancy, conceptual products, or functional alterations in maternal rats. Instituto Israelita de Ensino e Pesquisa Albert Einstein 2023-03-24 /pmc/articles/PMC10118372/ /pubmed/37075461 http://dx.doi.org/10.31744/einstein_journal/2023AO0230 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article de Moraes, Renata Delphim Araujo, Edward Castelo, Adauto Peixoto, Alberto Borges Amed, Abês Mahmed Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title | Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title_full | Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title_fullStr | Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title_full_unstemmed | Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title_short | Action of enfuvirtide on the pregnancy of albino rats ( Rattus norvegicus albinus, Rodentia, Mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
title_sort | action of enfuvirtide on the pregnancy of albino rats ( rattus norvegicus albinus, rodentia, mammalia ): biological assay and functional and histological analyses of exposed maternal-fetal organs |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118372/ https://www.ncbi.nlm.nih.gov/pubmed/37075461 http://dx.doi.org/10.31744/einstein_journal/2023AO0230 |
work_keys_str_mv | AT demoraesrenatadelphim actionofenfuvirtideonthepregnancyofalbinoratsrattusnorvegicusalbinusrodentiamammaliabiologicalassayandfunctionalandhistologicalanalysesofexposedmaternalfetalorgans AT araujoedward actionofenfuvirtideonthepregnancyofalbinoratsrattusnorvegicusalbinusrodentiamammaliabiologicalassayandfunctionalandhistologicalanalysesofexposedmaternalfetalorgans AT casteloadauto actionofenfuvirtideonthepregnancyofalbinoratsrattusnorvegicusalbinusrodentiamammaliabiologicalassayandfunctionalandhistologicalanalysesofexposedmaternalfetalorgans AT peixotoalbertoborges actionofenfuvirtideonthepregnancyofalbinoratsrattusnorvegicusalbinusrodentiamammaliabiologicalassayandfunctionalandhistologicalanalysesofexposedmaternalfetalorgans AT amedabesmahmed actionofenfuvirtideonthepregnancyofalbinoratsrattusnorvegicusalbinusrodentiamammaliabiologicalassayandfunctionalandhistologicalanalysesofexposedmaternalfetalorgans |