Expression of miR-155, miR-146a, and miR-326 in T1D patients from Chile: relationship with autoimmunity and inflammatory markers

OBJECTIVE: The aim of this research was to analyze the expression profile of miR-155, miR-146a, and miR-326 in peripheral blood mononuclear cells (PBMC) of 47 patients with type 1 diabetes mellitus (T1D) and 39 control subjects, as well as the possible association with autoimmune or inflammatory mar...

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Detalles Bibliográficos
Autores principales: García-Díaz, Diego F., Pizarro, Carolina, Camacho-Guillén, Patricia, Codner, Ethel, Soto, Néstor, Pérez-Bravo, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Endocrinologia e Metabologia 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118688/
https://www.ncbi.nlm.nih.gov/pubmed/29694627
http://dx.doi.org/10.20945/2359-3997000000006
Descripción
Sumario:OBJECTIVE: The aim of this research was to analyze the expression profile of miR-155, miR-146a, and miR-326 in peripheral blood mononuclear cells (PBMC) of 47 patients with type 1 diabetes mellitus (T1D) and 39 control subjects, as well as the possible association with autoimmune or inflammatory markers. SUBJECTS AND METHODS: Expression profile of miRs by means of qPCR using TaqMan probes. Autoantibodies and inflammatory markers by ELISA. Statistical analysis using bivariate correlation. RESULTS: The analysis of the results shows an increase in the expression of miR-155 in T1D patients in basal conditions compared to the controls (p < 0.001) and a decreased expression level of miR-326 (p < 0.01) and miR-146a (p < 0.05) compared T1D patients to the controls. miR-155 was the only miRs associated with autoinmmunity (ZnT8) and inflammatory status (vCAM). CONCLUSION: Our data show a possible role of miR-155 related to autoimmunity and inflammation in Chilean patients with T1D.

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