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Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
OBJECTIVE: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. Th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Endocrinologia e Metabologia
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118777/ https://www.ncbi.nlm.nih.gov/pubmed/29791662 http://dx.doi.org/10.20945/2359-3997000000044 |
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author | Cury, Sarah Santiloni Oliveira, Miriane Síbio, Maria Teresa Clara, Sueli Luvizotto, Renata De Azevedo Melo Conde, Sandro Jorge, Edson Nacib Nunes, Vania dos Santos Nogueira, Célia Regina Mazeto, Gláucia Maria Ferreira da Silva |
author_facet | Cury, Sarah Santiloni Oliveira, Miriane Síbio, Maria Teresa Clara, Sueli Luvizotto, Renata De Azevedo Melo Conde, Sandro Jorge, Edson Nacib Nunes, Vania dos Santos Nogueira, Célia Regina Mazeto, Gláucia Maria Ferreira da Silva |
author_sort | Cury, Sarah Santiloni |
collection | PubMed |
description | OBJECTIVE: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. MATERIALS AND METHODS: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). rRARα gene expression in the treated and untreated cells was then compared. RESULTS: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). CONCLUSIONS: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. |
format | Online Article Text |
id | pubmed-10118777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Sociedade Brasileira de Endocrinologia e Metabologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-101187772023-04-21 Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts Cury, Sarah Santiloni Oliveira, Miriane Síbio, Maria Teresa Clara, Sueli Luvizotto, Renata De Azevedo Melo Conde, Sandro Jorge, Edson Nacib Nunes, Vania dos Santos Nogueira, Célia Regina Mazeto, Gláucia Maria Ferreira da Silva Arch Endocrinol Metab Brief Report OBJECTIVE: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. MATERIALS AND METHODS: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). rRARα gene expression in the treated and untreated cells was then compared. RESULTS: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). CONCLUSIONS: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. Sociedade Brasileira de Endocrinologia e Metabologia 2018-05-07 /pmc/articles/PMC10118777/ /pubmed/29791662 http://dx.doi.org/10.20945/2359-3997000000044 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Cury, Sarah Santiloni Oliveira, Miriane Síbio, Maria Teresa Clara, Sueli Luvizotto, Renata De Azevedo Melo Conde, Sandro Jorge, Edson Nacib Nunes, Vania dos Santos Nogueira, Célia Regina Mazeto, Gláucia Maria Ferreira da Silva Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title | Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_full | Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_fullStr | Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_full_unstemmed | Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_short | Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_sort | graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118777/ https://www.ncbi.nlm.nih.gov/pubmed/29791662 http://dx.doi.org/10.20945/2359-3997000000044 |
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