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Diagnostic utility of DREAM gene mRNA levels in thyroid tumours
OBJECTIVE: The transcriptional repressor DREAM is involved in thyroid-specific gene expression, thyroid enlargement and nodular development, but its clinical utility is still uncertain. In this study we aimed to investigate whether DREAM mRNA levels differ in different thyroid tumors and how this po...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Endocrinologia e Metabologia
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118984/ https://www.ncbi.nlm.nih.gov/pubmed/29641740 http://dx.doi.org/10.20945/2359-3997000000028 |
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author | Batista, Fernando A. Marcello, Marjory A. Martins, Mariana B. Peres, Karina C. Cardoso, Ulieme O. Silva, Aline C. D. N. Bufalo, Natassia E. Soares, Fernando A. da Silva, Márcio J. Assumpção, Lígia V. Ward, Laura S. |
author_facet | Batista, Fernando A. Marcello, Marjory A. Martins, Mariana B. Peres, Karina C. Cardoso, Ulieme O. Silva, Aline C. D. N. Bufalo, Natassia E. Soares, Fernando A. da Silva, Márcio J. Assumpção, Lígia V. Ward, Laura S. |
author_sort | Batista, Fernando A. |
collection | PubMed |
description | OBJECTIVE: The transcriptional repressor DREAM is involved in thyroid-specific gene expression, thyroid enlargement and nodular development, but its clinical utility is still uncertain. In this study we aimed to investigate whether DREAM mRNA levels differ in different thyroid tumors and how this possible difference would allow the use of DREAM gene expression as molecular marker for diagnostic and/or prognosis purpose. MATERIALS AND METHODS: We quantified DREAM gene mRNA levels and investigated its mutational status, relating its expression and genetic changes to diagnostic and prognostic features of 200 thyroid tumors, being 101 malignant [99 papillary thyroid carcinomas (PTC) and 2 anaplastic thyroid carcinomas] and 99 benign thyroid lesions [49 goiter and 50 follicular adenomas (FA)]. RESULTS: Levels of mRNA of DREAM gene were higher in benign (0.7909 ± 0.6274 AU) than in malignant (0.3373 ± 0.6274 AU) thyroid lesions (p < 0.0001). DREAM gene expression was able to identify malignancy with 66.7% sensitivity, 85.4% specificity, 84.2% positive predictive value (PPV), 68.7% negative predictive value (NPV), and 75.3% accuracy. DREAM mRNA levels were also useful distinguishing the follicular lesions FA and FVPTC with 70.2% sensitivity, 73.5% specificity, 78.5% PPV, 64.1% NPV, and 71.6% accuracy. However, DREAM gene expression was neither associated with clinical features of tumor aggressiveness, nor with recurrence or survival. Six different genetic changes in non-coding regions of DREAM gene were also found, not related to DREAM gene expression or tumor features. CONCLUSION: We suggest that DREAM gene expression may help diagnose thyroid nodules, identifying malignancy and characterizing follicular-patterned thyroid lesions; however, it is not useful as a prognostic marker. |
format | Online Article Text |
id | pubmed-10118984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Sociedade Brasileira de Endocrinologia e Metabologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-101189842023-04-22 Diagnostic utility of DREAM gene mRNA levels in thyroid tumours Batista, Fernando A. Marcello, Marjory A. Martins, Mariana B. Peres, Karina C. Cardoso, Ulieme O. Silva, Aline C. D. N. Bufalo, Natassia E. Soares, Fernando A. da Silva, Márcio J. Assumpção, Lígia V. Ward, Laura S. Arch Endocrinol Metab Original Article OBJECTIVE: The transcriptional repressor DREAM is involved in thyroid-specific gene expression, thyroid enlargement and nodular development, but its clinical utility is still uncertain. In this study we aimed to investigate whether DREAM mRNA levels differ in different thyroid tumors and how this possible difference would allow the use of DREAM gene expression as molecular marker for diagnostic and/or prognosis purpose. MATERIALS AND METHODS: We quantified DREAM gene mRNA levels and investigated its mutational status, relating its expression and genetic changes to diagnostic and prognostic features of 200 thyroid tumors, being 101 malignant [99 papillary thyroid carcinomas (PTC) and 2 anaplastic thyroid carcinomas] and 99 benign thyroid lesions [49 goiter and 50 follicular adenomas (FA)]. RESULTS: Levels of mRNA of DREAM gene were higher in benign (0.7909 ± 0.6274 AU) than in malignant (0.3373 ± 0.6274 AU) thyroid lesions (p < 0.0001). DREAM gene expression was able to identify malignancy with 66.7% sensitivity, 85.4% specificity, 84.2% positive predictive value (PPV), 68.7% negative predictive value (NPV), and 75.3% accuracy. DREAM mRNA levels were also useful distinguishing the follicular lesions FA and FVPTC with 70.2% sensitivity, 73.5% specificity, 78.5% PPV, 64.1% NPV, and 71.6% accuracy. However, DREAM gene expression was neither associated with clinical features of tumor aggressiveness, nor with recurrence or survival. Six different genetic changes in non-coding regions of DREAM gene were also found, not related to DREAM gene expression or tumor features. CONCLUSION: We suggest that DREAM gene expression may help diagnose thyroid nodules, identifying malignancy and characterizing follicular-patterned thyroid lesions; however, it is not useful as a prognostic marker. Sociedade Brasileira de Endocrinologia e Metabologia 2018-03-23 /pmc/articles/PMC10118984/ /pubmed/29641740 http://dx.doi.org/10.20945/2359-3997000000028 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Batista, Fernando A. Marcello, Marjory A. Martins, Mariana B. Peres, Karina C. Cardoso, Ulieme O. Silva, Aline C. D. N. Bufalo, Natassia E. Soares, Fernando A. da Silva, Márcio J. Assumpção, Lígia V. Ward, Laura S. Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title | Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title_full | Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title_fullStr | Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title_full_unstemmed | Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title_short | Diagnostic utility of DREAM gene mRNA levels in thyroid tumours |
title_sort | diagnostic utility of dream gene mrna levels in thyroid tumours |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118984/ https://www.ncbi.nlm.nih.gov/pubmed/29641740 http://dx.doi.org/10.20945/2359-3997000000028 |
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