Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium

Vascular cambium contains bifacial stem cells, which produce secondary xylem to one side and secondary phloem to the other. However, how these fate decisions are regulated is unknown. Here we show that the positioning of an auxin signalling maximum within the cambium determines the fate of stem cell...

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Autores principales: Mäkilä, Riikka, Wybouw, Brecht, Smetana, Ondřej, Vainio, Leo, Solé-Gil, Anna, Lyu, Munan, Ye, Lingling, Wang, Xin, Siligato, Riccardo, Jenness, Mark K., Murphy, Angus S., Mähönen, Ari Pekka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119023/
https://www.ncbi.nlm.nih.gov/pubmed/36997686
http://dx.doi.org/10.1038/s41477-023-01360-w
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author Mäkilä, Riikka
Wybouw, Brecht
Smetana, Ondřej
Vainio, Leo
Solé-Gil, Anna
Lyu, Munan
Ye, Lingling
Wang, Xin
Siligato, Riccardo
Jenness, Mark K.
Murphy, Angus S.
Mähönen, Ari Pekka
author_facet Mäkilä, Riikka
Wybouw, Brecht
Smetana, Ondřej
Vainio, Leo
Solé-Gil, Anna
Lyu, Munan
Ye, Lingling
Wang, Xin
Siligato, Riccardo
Jenness, Mark K.
Murphy, Angus S.
Mähönen, Ari Pekka
author_sort Mäkilä, Riikka
collection PubMed
description Vascular cambium contains bifacial stem cells, which produce secondary xylem to one side and secondary phloem to the other. However, how these fate decisions are regulated is unknown. Here we show that the positioning of an auxin signalling maximum within the cambium determines the fate of stem cell daughters. The position is modulated by gibberellin-regulated, PIN1-dependent polar auxin transport. Gibberellin treatment broadens auxin maximum from the xylem side of the cambium towards the phloem. As a result, xylem-side stem cell daughter preferentially differentiates into xylem, while phloem-side daughter retains stem cell identity. Occasionally, this broadening leads to direct specification of both daughters as xylem, and consequently, adjacent phloem-identity cell reverts to being stem cell. Conversely, reduced gibberellin levels favour specification of phloem-side stem cell daughter as phloem. Together, our data provide a mechanism by which gibberellin regulates the ratio of xylem and phloem production.
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spelling pubmed-101190232023-04-22 Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium Mäkilä, Riikka Wybouw, Brecht Smetana, Ondřej Vainio, Leo Solé-Gil, Anna Lyu, Munan Ye, Lingling Wang, Xin Siligato, Riccardo Jenness, Mark K. Murphy, Angus S. Mähönen, Ari Pekka Nat Plants Article Vascular cambium contains bifacial stem cells, which produce secondary xylem to one side and secondary phloem to the other. However, how these fate decisions are regulated is unknown. Here we show that the positioning of an auxin signalling maximum within the cambium determines the fate of stem cell daughters. The position is modulated by gibberellin-regulated, PIN1-dependent polar auxin transport. Gibberellin treatment broadens auxin maximum from the xylem side of the cambium towards the phloem. As a result, xylem-side stem cell daughter preferentially differentiates into xylem, while phloem-side daughter retains stem cell identity. Occasionally, this broadening leads to direct specification of both daughters as xylem, and consequently, adjacent phloem-identity cell reverts to being stem cell. Conversely, reduced gibberellin levels favour specification of phloem-side stem cell daughter as phloem. Together, our data provide a mechanism by which gibberellin regulates the ratio of xylem and phloem production. Nature Publishing Group UK 2023-03-30 2023 /pmc/articles/PMC10119023/ /pubmed/36997686 http://dx.doi.org/10.1038/s41477-023-01360-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mäkilä, Riikka
Wybouw, Brecht
Smetana, Ondřej
Vainio, Leo
Solé-Gil, Anna
Lyu, Munan
Ye, Lingling
Wang, Xin
Siligato, Riccardo
Jenness, Mark K.
Murphy, Angus S.
Mähönen, Ari Pekka
Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title_full Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title_fullStr Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title_full_unstemmed Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title_short Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
title_sort gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119023/
https://www.ncbi.nlm.nih.gov/pubmed/36997686
http://dx.doi.org/10.1038/s41477-023-01360-w
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