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Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia

Lung adenocarcinoma is still cancer with the highest mortality. Hypoxia and immunity play an essential role in the occurrence and development of tumors. Therefore, this study is mainly to find new early diagnosis and prognosis markers and explore the relationship among the markers and immunity and h...

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Autores principales: Sun, Kang, Zhang, Zhiqiang, Wang, Dongqin, Huang, Yinlong, Zhang, Jing, Lian, Chaoqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119119/
https://www.ncbi.nlm.nih.gov/pubmed/37081097
http://dx.doi.org/10.1038/s41598-023-33404-8
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author Sun, Kang
Zhang, Zhiqiang
Wang, Dongqin
Huang, Yinlong
Zhang, Jing
Lian, Chaoqun
author_facet Sun, Kang
Zhang, Zhiqiang
Wang, Dongqin
Huang, Yinlong
Zhang, Jing
Lian, Chaoqun
author_sort Sun, Kang
collection PubMed
description Lung adenocarcinoma is still cancer with the highest mortality. Hypoxia and immunity play an essential role in the occurrence and development of tumors. Therefore, this study is mainly to find new early diagnosis and prognosis markers and explore the relationship among the markers and immunity and hypoxia, to improve the prognosis of patients. Firstly, based on the clinical database in TCGA, we determined the most critical clinicopathological parameters affecting the prognosis of patients through a variety of analysis methods. According to pathological parameters, logistic most minor absolute contraction selection operator (lasso), univariate and multivariate regression analysis, the risk genes related to early prognosis were screened, and the risk model was established. Then, in different risk groups, GSEA and CIBERSORT algorithms were used to analyze the distribution and enrichment of the immune cells and hypoxia, to study the effects of early prognostic indicators on hypoxia and immunity. At the same time, we analyzed the different levels of risk genes in normal cells (BSEA-2B) and tumor cells (H1299, A549, PC9, and H1975). Finally, A549 and PC9 cells were induced by CoCl2 to establish a hypoxic environment, and the correlation between risk genes and HIF1A was analyzed. The risk model based on risk genes (CYP4B1, KRT6A, and FAM83A) was accurate and stable for the prognosis of patients. It is closely related to immunity and hypoxia. In BSEA-2B cells, the mRNA and protein expression of CYP4B1 was higher, while the expression of KRT6A and FAM83A was lower. Finally, we found that FAM83A and HIF1A showed a significant positive correlation when A549 and PC9 cells were exposed to hypoxia. The discovery of early diagnostic markers related to immunity, hypoxia, and prognosis, provides a new idea for early screening and prognostic treatment of lung adenocarcinoma.
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spelling pubmed-101191192023-04-22 Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia Sun, Kang Zhang, Zhiqiang Wang, Dongqin Huang, Yinlong Zhang, Jing Lian, Chaoqun Sci Rep Article Lung adenocarcinoma is still cancer with the highest mortality. Hypoxia and immunity play an essential role in the occurrence and development of tumors. Therefore, this study is mainly to find new early diagnosis and prognosis markers and explore the relationship among the markers and immunity and hypoxia, to improve the prognosis of patients. Firstly, based on the clinical database in TCGA, we determined the most critical clinicopathological parameters affecting the prognosis of patients through a variety of analysis methods. According to pathological parameters, logistic most minor absolute contraction selection operator (lasso), univariate and multivariate regression analysis, the risk genes related to early prognosis were screened, and the risk model was established. Then, in different risk groups, GSEA and CIBERSORT algorithms were used to analyze the distribution and enrichment of the immune cells and hypoxia, to study the effects of early prognostic indicators on hypoxia and immunity. At the same time, we analyzed the different levels of risk genes in normal cells (BSEA-2B) and tumor cells (H1299, A549, PC9, and H1975). Finally, A549 and PC9 cells were induced by CoCl2 to establish a hypoxic environment, and the correlation between risk genes and HIF1A was analyzed. The risk model based on risk genes (CYP4B1, KRT6A, and FAM83A) was accurate and stable for the prognosis of patients. It is closely related to immunity and hypoxia. In BSEA-2B cells, the mRNA and protein expression of CYP4B1 was higher, while the expression of KRT6A and FAM83A was lower. Finally, we found that FAM83A and HIF1A showed a significant positive correlation when A549 and PC9 cells were exposed to hypoxia. The discovery of early diagnostic markers related to immunity, hypoxia, and prognosis, provides a new idea for early screening and prognostic treatment of lung adenocarcinoma. Nature Publishing Group UK 2023-04-20 /pmc/articles/PMC10119119/ /pubmed/37081097 http://dx.doi.org/10.1038/s41598-023-33404-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sun, Kang
Zhang, Zhiqiang
Wang, Dongqin
Huang, Yinlong
Zhang, Jing
Lian, Chaoqun
Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title_full Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title_fullStr Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title_full_unstemmed Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title_short Regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
title_sort regulation of early diagnosis and prognostic markers of lung adenocarcinoma in immunity and hypoxia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119119/
https://www.ncbi.nlm.nih.gov/pubmed/37081097
http://dx.doi.org/10.1038/s41598-023-33404-8
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