Cargando…
Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate
Longitudinal bone growth relies on endochondral ossification in the cartilaginous growth plate, where chondrocytes accumulate and synthesize the matrix scaffold that is replaced by bone. The chondroprogenitors in the resting zone maintain the continuous turnover of chondrocytes in the growth plate....
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119120/ https://www.ncbi.nlm.nih.gov/pubmed/37080994 http://dx.doi.org/10.1038/s41413-023-00258-9 |
_version_ | 1785028955319828480 |
---|---|
author | Oichi, Takeshi Kodama, Joe Wilson, Kimberly Tian, Hongying Imamura Kawasawa, Yuka Usami, Yu Oshima, Yasushi Saito, Taku Tanaka, Sakae Iwamoto, Masahiro Otsuru, Satoru Enomoto-Iwamoto, Motomi |
author_facet | Oichi, Takeshi Kodama, Joe Wilson, Kimberly Tian, Hongying Imamura Kawasawa, Yuka Usami, Yu Oshima, Yasushi Saito, Taku Tanaka, Sakae Iwamoto, Masahiro Otsuru, Satoru Enomoto-Iwamoto, Motomi |
author_sort | Oichi, Takeshi |
collection | PubMed |
description | Longitudinal bone growth relies on endochondral ossification in the cartilaginous growth plate, where chondrocytes accumulate and synthesize the matrix scaffold that is replaced by bone. The chondroprogenitors in the resting zone maintain the continuous turnover of chondrocytes in the growth plate. Malnutrition is a leading cause of growth retardation in children; however, after recovery from nutrient deprivation, bone growth is accelerated beyond the normal rate, a phenomenon termed catch-up growth. Although nutritional status is a known regulator of long bone growth, it is largely unknown whether and how chondroprogenitor cells respond to deviations in nutrient availability. Here, using fate-mapping analysis in Axin2Cre(ERT2) mice, we showed that dietary restriction increased the number of Axin2(+) chondroprogenitors in the resting zone and simultaneously inhibited their differentiation. Once nutrient deficiency was resolved, the accumulated chondroprogenitor cells immediately restarted differentiation and formed chondrocyte columns, contributing to accelerated growth. Furthermore, we showed that nutrient deprivation reduced the level of phosphorylated Akt in the resting zone and that exogenous IGF-1 restored the phosphorylated Akt level and stimulated differentiation of the pooled chondroprogenitors, decreasing their numbers. Our study of Axin2Cre(ERT2) revealed that nutrient availability regulates the balance between accumulation and differentiation of chondroprogenitors in the growth plate and further demonstrated that IGF-1 partially mediates this regulation by promoting the committed differentiation of chondroprogenitor cells. |
format | Online Article Text |
id | pubmed-10119120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101191202023-04-22 Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate Oichi, Takeshi Kodama, Joe Wilson, Kimberly Tian, Hongying Imamura Kawasawa, Yuka Usami, Yu Oshima, Yasushi Saito, Taku Tanaka, Sakae Iwamoto, Masahiro Otsuru, Satoru Enomoto-Iwamoto, Motomi Bone Res Article Longitudinal bone growth relies on endochondral ossification in the cartilaginous growth plate, where chondrocytes accumulate and synthesize the matrix scaffold that is replaced by bone. The chondroprogenitors in the resting zone maintain the continuous turnover of chondrocytes in the growth plate. Malnutrition is a leading cause of growth retardation in children; however, after recovery from nutrient deprivation, bone growth is accelerated beyond the normal rate, a phenomenon termed catch-up growth. Although nutritional status is a known regulator of long bone growth, it is largely unknown whether and how chondroprogenitor cells respond to deviations in nutrient availability. Here, using fate-mapping analysis in Axin2Cre(ERT2) mice, we showed that dietary restriction increased the number of Axin2(+) chondroprogenitors in the resting zone and simultaneously inhibited their differentiation. Once nutrient deficiency was resolved, the accumulated chondroprogenitor cells immediately restarted differentiation and formed chondrocyte columns, contributing to accelerated growth. Furthermore, we showed that nutrient deprivation reduced the level of phosphorylated Akt in the resting zone and that exogenous IGF-1 restored the phosphorylated Akt level and stimulated differentiation of the pooled chondroprogenitors, decreasing their numbers. Our study of Axin2Cre(ERT2) revealed that nutrient availability regulates the balance between accumulation and differentiation of chondroprogenitors in the growth plate and further demonstrated that IGF-1 partially mediates this regulation by promoting the committed differentiation of chondroprogenitor cells. Nature Publishing Group UK 2023-04-21 /pmc/articles/PMC10119120/ /pubmed/37080994 http://dx.doi.org/10.1038/s41413-023-00258-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Oichi, Takeshi Kodama, Joe Wilson, Kimberly Tian, Hongying Imamura Kawasawa, Yuka Usami, Yu Oshima, Yasushi Saito, Taku Tanaka, Sakae Iwamoto, Masahiro Otsuru, Satoru Enomoto-Iwamoto, Motomi Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title | Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title_full | Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title_fullStr | Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title_full_unstemmed | Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title_short | Nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
title_sort | nutrient-regulated dynamics of chondroprogenitors in the postnatal murine growth plate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119120/ https://www.ncbi.nlm.nih.gov/pubmed/37080994 http://dx.doi.org/10.1038/s41413-023-00258-9 |
work_keys_str_mv | AT oichitakeshi nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT kodamajoe nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT wilsonkimberly nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT tianhongying nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT imamurakawasawayuka nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT usamiyu nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT oshimayasushi nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT saitotaku nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT tanakasakae nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT iwamotomasahiro nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT otsurusatoru nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate AT enomotoiwamotomotomi nutrientregulateddynamicsofchondroprogenitorsinthepostnatalmurinegrowthplate |