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Bee venom as an alternative for antibiotics against Staphylococcus aureus infections
The misuse of antibiotics has led to antibiotic-resistant bacterial strains, making it even harder to combat and eliminate their infections. Staphylococcus aureus causes various adverse infections and diseases, including skin abscesses, bloodstream infections, pneumonia, and joint infections. In thi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119156/ https://www.ncbi.nlm.nih.gov/pubmed/37081055 http://dx.doi.org/10.1038/s41598-023-33536-x |
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author | Sameh, Ahmed Gouda, Amr A. Elmligy, Esraa Hatem, Hossam Sadek, Salma S. Ahmed, Osama El Amir, Azza |
author_facet | Sameh, Ahmed Gouda, Amr A. Elmligy, Esraa Hatem, Hossam Sadek, Salma S. Ahmed, Osama El Amir, Azza |
author_sort | Sameh, Ahmed |
collection | PubMed |
description | The misuse of antibiotics has led to antibiotic-resistant bacterial strains, making it even harder to combat and eliminate their infections. Staphylococcus aureus causes various adverse infections and diseases, including skin abscesses, bloodstream infections, pneumonia, and joint infections. In this study, we aimed to test the cytotoxic and antibacterial effects of bee venom-loaded chitosan nanoparticles (BV-loaded CS-NPs) in comparison to gamma-irradiated BV and native BV from Apis mellifera. The physiochemical characterizations of our treatments were determined by Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscope (TEM), zeta-potential, release rate, and Encapsulation Efficiency (EE). Our study was conducted on both levels, in-vitro and in-vivo. For the in-vitro study, a bacterial model of Staphylococcus aureus with an ATCC number of 6538 was grown in tryptic soy agar (TSA) medium, and the inhibition zones of our drug candidates were measured with the appropriate statistical analysis performed. For the in-vivo study, levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), Creatinine, Urea, and interleukin 6 (IL-6) were analyzed. BV-loaded CS-NPs showed relatively better results than the other alternatives, which are native BV and gamma-irradiated BV. The results showed that the antibacterial effect of BV-loaded CS-NPs was greater than the alternatives. Furthermore, its cytotoxic effect was far less than the native and irradiated bee venom. These outcomes ensure that loading BV on CS-NPs makes it a promising drug candidate for an antibiotic alternative with minimal cytotoxicity and enhanced antibacterial activity. |
format | Online Article Text |
id | pubmed-10119156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101191562023-04-22 Bee venom as an alternative for antibiotics against Staphylococcus aureus infections Sameh, Ahmed Gouda, Amr A. Elmligy, Esraa Hatem, Hossam Sadek, Salma S. Ahmed, Osama El Amir, Azza Sci Rep Article The misuse of antibiotics has led to antibiotic-resistant bacterial strains, making it even harder to combat and eliminate their infections. Staphylococcus aureus causes various adverse infections and diseases, including skin abscesses, bloodstream infections, pneumonia, and joint infections. In this study, we aimed to test the cytotoxic and antibacterial effects of bee venom-loaded chitosan nanoparticles (BV-loaded CS-NPs) in comparison to gamma-irradiated BV and native BV from Apis mellifera. The physiochemical characterizations of our treatments were determined by Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscope (TEM), zeta-potential, release rate, and Encapsulation Efficiency (EE). Our study was conducted on both levels, in-vitro and in-vivo. For the in-vitro study, a bacterial model of Staphylococcus aureus with an ATCC number of 6538 was grown in tryptic soy agar (TSA) medium, and the inhibition zones of our drug candidates were measured with the appropriate statistical analysis performed. For the in-vivo study, levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), Creatinine, Urea, and interleukin 6 (IL-6) were analyzed. BV-loaded CS-NPs showed relatively better results than the other alternatives, which are native BV and gamma-irradiated BV. The results showed that the antibacterial effect of BV-loaded CS-NPs was greater than the alternatives. Furthermore, its cytotoxic effect was far less than the native and irradiated bee venom. These outcomes ensure that loading BV on CS-NPs makes it a promising drug candidate for an antibiotic alternative with minimal cytotoxicity and enhanced antibacterial activity. Nature Publishing Group UK 2023-04-20 /pmc/articles/PMC10119156/ /pubmed/37081055 http://dx.doi.org/10.1038/s41598-023-33536-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sameh, Ahmed Gouda, Amr A. Elmligy, Esraa Hatem, Hossam Sadek, Salma S. Ahmed, Osama El Amir, Azza Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title | Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title_full | Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title_fullStr | Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title_full_unstemmed | Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title_short | Bee venom as an alternative for antibiotics against Staphylococcus aureus infections |
title_sort | bee venom as an alternative for antibiotics against staphylococcus aureus infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119156/ https://www.ncbi.nlm.nih.gov/pubmed/37081055 http://dx.doi.org/10.1038/s41598-023-33536-x |
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