Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine

[Image: see text] A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP)...

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Autores principales: Chen, Wei-Hsin, Wang, Wenting, Lin, Qianqi, Grys, David-Benjamin, Niihori, Marika, Huang, Junyang, Hu, Shu, de Nijs, Bart, Scherman, Oren A., Baumberg, Jeremy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119978/
https://www.ncbi.nlm.nih.gov/pubmed/37096231
http://dx.doi.org/10.1021/acsnanoscienceau.2c00048
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author Chen, Wei-Hsin
Wang, Wenting
Lin, Qianqi
Grys, David-Benjamin
Niihori, Marika
Huang, Junyang
Hu, Shu
de Nijs, Bart
Scherman, Oren A.
Baumberg, Jeremy J.
author_facet Chen, Wei-Hsin
Wang, Wenting
Lin, Qianqi
Grys, David-Benjamin
Niihori, Marika
Huang, Junyang
Hu, Shu
de Nijs, Bart
Scherman, Oren A.
Baumberg, Jeremy J.
author_sort Chen, Wei-Hsin
collection PubMed
description [Image: see text] A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) “mix-and-measure” protocols, in which Fe(III) bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis.
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spelling pubmed-101199782023-04-22 Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine Chen, Wei-Hsin Wang, Wenting Lin, Qianqi Grys, David-Benjamin Niihori, Marika Huang, Junyang Hu, Shu de Nijs, Bart Scherman, Oren A. Baumberg, Jeremy J. ACS Nanosci Au [Image: see text] A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) “mix-and-measure” protocols, in which Fe(III) bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis. American Chemical Society 2022-12-24 /pmc/articles/PMC10119978/ /pubmed/37096231 http://dx.doi.org/10.1021/acsnanoscienceau.2c00048 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Chen, Wei-Hsin
Wang, Wenting
Lin, Qianqi
Grys, David-Benjamin
Niihori, Marika
Huang, Junyang
Hu, Shu
de Nijs, Bart
Scherman, Oren A.
Baumberg, Jeremy J.
Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title_full Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title_fullStr Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title_full_unstemmed Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title_short Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine
title_sort plasmonic sensing assay for long-term monitoring (psalm) of neurotransmitters in urine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119978/
https://www.ncbi.nlm.nih.gov/pubmed/37096231
http://dx.doi.org/10.1021/acsnanoscienceau.2c00048
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