Cargando…
Novel Experimental and Analysis Strategies for Fast Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations
[Image: see text] Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and gen...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120031/ https://www.ncbi.nlm.nih.gov/pubmed/37090258 http://dx.doi.org/10.1021/acsmeasuresciau.2c00059 |
_version_ | 1785029111343742976 |
---|---|
author | Hexter, Melissa van Batenburg-Sherwood, Joseph Hashemi, Parastoo |
author_facet | Hexter, Melissa van Batenburg-Sherwood, Joseph Hashemi, Parastoo |
author_sort | Hexter, Melissa |
collection | PubMed |
description | [Image: see text] Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and generally employs flow injection analysis (FIA) performed with flow cells fabricated in-house. Such FSCV FIA cells often require significant and ongoing troubleshooting with pulsing, leaking, flow inconsistencies and dead volume being major causes of common challenges. In this work, we address these issues by creating a robust, plug-and-play FSCV flow cell. This novel design permits reproducible, high-precision, and stable flow injection profiles using low-cost materials to improve FSCV calibration. The ready-to-print computer-aided designs and hardware list are provided. |
format | Online Article Text |
id | pubmed-10120031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-101200312023-04-22 Novel Experimental and Analysis Strategies for Fast Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations Hexter, Melissa van Batenburg-Sherwood, Joseph Hashemi, Parastoo ACS Meas Sci Au [Image: see text] Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and generally employs flow injection analysis (FIA) performed with flow cells fabricated in-house. Such FSCV FIA cells often require significant and ongoing troubleshooting with pulsing, leaking, flow inconsistencies and dead volume being major causes of common challenges. In this work, we address these issues by creating a robust, plug-and-play FSCV flow cell. This novel design permits reproducible, high-precision, and stable flow injection profiles using low-cost materials to improve FSCV calibration. The ready-to-print computer-aided designs and hardware list are provided. American Chemical Society 2023-01-11 /pmc/articles/PMC10120031/ /pubmed/37090258 http://dx.doi.org/10.1021/acsmeasuresciau.2c00059 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Hexter, Melissa van Batenburg-Sherwood, Joseph Hashemi, Parastoo Novel Experimental and Analysis Strategies for Fast Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title | Novel Experimental
and Analysis Strategies for Fast
Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title_full | Novel Experimental
and Analysis Strategies for Fast
Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title_fullStr | Novel Experimental
and Analysis Strategies for Fast
Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title_full_unstemmed | Novel Experimental
and Analysis Strategies for Fast
Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title_short | Novel Experimental
and Analysis Strategies for Fast
Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV Calibrations |
title_sort | novel experimental
and analysis strategies for fast
voltammetry: 2. a troubleshoot-free flow cell for fscv calibrations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120031/ https://www.ncbi.nlm.nih.gov/pubmed/37090258 http://dx.doi.org/10.1021/acsmeasuresciau.2c00059 |
work_keys_str_mv | AT hextermelissa novelexperimentalandanalysisstrategiesforfastvoltammetry2atroubleshootfreeflowcellforfscvcalibrations AT vanbatenburgsherwoodjoseph novelexperimentalandanalysisstrategiesforfastvoltammetry2atroubleshootfreeflowcellforfscvcalibrations AT hashemiparastoo novelexperimentalandanalysisstrategiesforfastvoltammetry2atroubleshootfreeflowcellforfscvcalibrations |