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Single-shot time-folded fluorescence lifetime imaging
Fluorescence lifetime imaging is an important tool in bioimaging that allows one to detect subtle changes in cell dynamics and their environment. Most time-domain approaches currently involve scanning a single illumination point across the sample, which can make imaging dynamic scenes challenging, w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120087/ https://www.ncbi.nlm.nih.gov/pubmed/37043531 http://dx.doi.org/10.1073/pnas.2214617120 |
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author | Kapitany, Valentin Zickus, Vytautas Fatima, Areeba Carles, Guillem Faccio, Daniele |
author_facet | Kapitany, Valentin Zickus, Vytautas Fatima, Areeba Carles, Guillem Faccio, Daniele |
author_sort | Kapitany, Valentin |
collection | PubMed |
description | Fluorescence lifetime imaging is an important tool in bioimaging that allows one to detect subtle changes in cell dynamics and their environment. Most time-domain approaches currently involve scanning a single illumination point across the sample, which can make imaging dynamic scenes challenging, while single-shot “rapid lifetime determination” can suffer from large uncertainties when the lifetime is not appropriately sampled. Here, we propose a time-folded fluorescence lifetime imaging microscopy (TFFLIM) approach, whereby a time-folding cavity provides multiple spatially sheared replicas of the lifetime, each shifted temporally with respect to a fixed time gate. This provides a robust, single-shot FLIM approach that we experimentally validate across a broad lifetime range on fluorescent beads and Convallaria samples. |
format | Online Article Text |
id | pubmed-10120087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-101200872023-04-22 Single-shot time-folded fluorescence lifetime imaging Kapitany, Valentin Zickus, Vytautas Fatima, Areeba Carles, Guillem Faccio, Daniele Proc Natl Acad Sci U S A Biological Sciences Fluorescence lifetime imaging is an important tool in bioimaging that allows one to detect subtle changes in cell dynamics and their environment. Most time-domain approaches currently involve scanning a single illumination point across the sample, which can make imaging dynamic scenes challenging, while single-shot “rapid lifetime determination” can suffer from large uncertainties when the lifetime is not appropriately sampled. Here, we propose a time-folded fluorescence lifetime imaging microscopy (TFFLIM) approach, whereby a time-folding cavity provides multiple spatially sheared replicas of the lifetime, each shifted temporally with respect to a fixed time gate. This provides a robust, single-shot FLIM approach that we experimentally validate across a broad lifetime range on fluorescent beads and Convallaria samples. National Academy of Sciences 2023-04-12 2023-04-18 /pmc/articles/PMC10120087/ /pubmed/37043531 http://dx.doi.org/10.1073/pnas.2214617120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biological Sciences Kapitany, Valentin Zickus, Vytautas Fatima, Areeba Carles, Guillem Faccio, Daniele Single-shot time-folded fluorescence lifetime imaging |
title | Single-shot time-folded fluorescence lifetime imaging |
title_full | Single-shot time-folded fluorescence lifetime imaging |
title_fullStr | Single-shot time-folded fluorescence lifetime imaging |
title_full_unstemmed | Single-shot time-folded fluorescence lifetime imaging |
title_short | Single-shot time-folded fluorescence lifetime imaging |
title_sort | single-shot time-folded fluorescence lifetime imaging |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120087/ https://www.ncbi.nlm.nih.gov/pubmed/37043531 http://dx.doi.org/10.1073/pnas.2214617120 |
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